Release of periplasmic proteins of Brucella suis upon acidic shock involves the outer membrane protein Omp25

Infection and Immunity
Rose-Anne BoigegrainBruno Rouot

Abstract

The survival and replication of Brucella in macrophages is initially triggered by a low intraphagosomal pH. In order to identify proteins released by Brucella during this early acidification step, we analyzed Brucella suis conditioned medium at various pH levels. No significant proteins were released at pH 4.0 in minimal medium or citrate buffer, whereas in acetate buffer, B. suis released a substantial amount of soluble proteins. Comparison of 13 N-terminal amino acid sequences determined by Edman degradation with their corresponding genomic sequences revealed that all of these proteins possessed a signal peptide indicative of their periplasmic location. Ten proteins are putative substrate binding proteins, including a homologue of the nopaline binding protein of Agrobacterium tumefaciens. The absence of this homologue in Brucella melitensis was due to the deletion of a 7.7-kb DNA fragment in its genome. We also characterized for the first time a hypothetical 9.8-kDa basic protein composed of five amino acid repeats. In B. suis, this protein contained 9 repeats, while 12 were present in the B. melitensis orthologue. B. suis in acetate buffer depended on neither the virB type IV secretory system nor the omp31 gene product. Howe...Continue Reading

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Citations

Jun 15, 2007·Infection and Immunity·Paola Caro-HernándezNieves Vizcaíno
Jul 19, 2013·BioMed Research International·Eric Daniel Avila-CalderónAraceli Contreras-Rodríguez
Jan 14, 2012·Clinical & Developmental Immunology·Eric Daniel Avila-CalderónAraceli Contreras-Rodríguez
May 26, 2010·FEMS Immunology and Medical Microbiology·Marie GodefroidJean-Jacques Letesson
Jun 15, 2007·FEMS Immunology and Medical Microbiology·Anita P O GodoyJosé Pedrazzoli
Oct 9, 2014·Archives of Microbiology·Eric Daniel Avila-CalderónAraceli Contreras-Rodríguez
Apr 26, 2020·Proteomes·Ansgar Poetsch, María Inés Marchesini
Dec 19, 2019·Frontiers in Microbiology·Minerva Araiza-VillanuevaAraceli Contreras-Rodríguez
Jan 15, 2017·Journal of Chromatographic Science·Bilal AslamMuhammad Hidayat Rasool
Jan 13, 2021·Archives of Microbiology·María Del Socorro Ruiz-PalmaAraceli Contreras-Rodríguez
Mar 9, 2007·Journal of Proteome Research·Julie LamontagneEustache Paramithiotis

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Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

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Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.