Jun 1, 1974

Release of polarity in Escherichia coli by gene N of phage lambda: termination and antitermination of transcription

Proceedings of the National Academy of Sciences of the United States of America
S AdhyaB de Crombrugghe


The induction of lambda prophage provokes the constitutive expression of the adjacent gal operon in E. coli. This "escape synthesis" can result from transcription that initiates at a phage promoter and extends into the gal operon. The effect requires the product of the lambda gene N. N-mediated transcription not only fails to terminate at the prophage-bacterial junction and at the ends of bacterial operons, but ignores termination signals caused by polar insertions or ochre mutations within gal. Suppression of polarity by N-function is a cis-effect; only transcription initiated at the phage promoter is influenced. We propose that the transcription complex is influenced by N-product to become termination-resistant at a site in the phage genome (juggernaut model). This site appears to be at or near the phage promoter.

Mentioned in this Paper

Alkalescens-Dispar Group
Transcription, Genetic
Genome Mapping
Visual Suppression
Genes, Regulator

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