Release of proteinase from mycelium of Mucor hiemalis.

Journal of Bacteriology
H L Wang

Abstract

When Mucor hiemalis NRRL 3103 was grown in soybean medium, only a small fraction of the proteinase produced by the organism appeared in the culture filtrate, whereas the bulk of the enzyme was bound to the mycelial surface. Optimal pH of the proteinase ranged from 3.0 to 3.5. Inclusion of sodium chloride or other ionizable salts in the growth medium, however, resulted in the liberation from the mycelium of the loosely bound enzyme as it was formed. Maximal release of proteinase was achieved at a sodium chloride concentration of 0.5 m. The loosely bound proteinase was eluted also from intact resting mycelium by ionizable salts but not by water or by nonionizable substances. The amount of enzyme eluted from the mycelium depended upon the concentration of sodium chloride up to 0.3 m. Since liberation took place rapidly even at 0 C, a loose ionic linkage must exist rather than a biochemical binding of the enzyme to the mycelium. The recovery of proteolytic activity from repeated salt extractions was greater than that originally detected in the intact mycelium, possibly owing to unmasking of more active enzymes or functional groups. Further proteinase activity was released when salt-extracted mycelium was ruptured. Part of the prote...Continue Reading

References

Oct 14, 1964·Biochemical and Biophysical Research Communications·H C Neu, L A Heppel
Jan 1, 1964·Biochemical and Biophysical Research Communications·H C Neu, L A Heppel
Jan 1, 1966·Journal of Bacteriology·R Weimberg, W L Orton
Aug 1, 1956·Journal of General Microbiology·M R POLLOCK
Dec 1, 1958·The Biochemical Journal·M R POLLOCK, M KRAMER
Jun 2, 1961·Biochemical and Biophysical Research Communications·M MALAMY, B L HORECKER
Oct 1, 1961·Journal of General Microbiology·D J KUSHNER, M R POLLOCK

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Citations

Jun 18, 2010·Environmental Microbiology·Eliane FrimmersdorfDietmar Schomburg
May 1, 1992·World Journal of Microbiology & Biotechnology·M V Deshpande
Mar 24, 2009·Journal of Bacteriology·Amalia RocaJuan L Ramos
Apr 1, 1975·Genetical Research·M Polkinghorne, M J Hynes
Dec 4, 2010·Applied Microbiology and Biotechnology·Sirma YeginCanan Tari
Feb 24, 2001·Critical Reviews in Biotechnology·X Shi, D Y Fung
Aug 31, 2012·Microbiology and Molecular Biology Reviews : MMBR·Robert A Bender
Oct 1, 1970·Journal of Bacteriology·C P Newell, T G Lessie
Feb 1, 1989·Journal of Bacteriology·T A Kroening, K E Kendrick
Feb 1, 1995·Journal of Bacteriology·P C WuK E Kendrick
Aug 1, 1968·Journal of Bacteriology·D H HugJ Hunter
Aug 1, 1970·Journal of Bacteriology·M J Hynes
Apr 1, 1974·Applied Microbiology·T ShibataniI Chibata
Nov 1, 1968·Comparative Biochemistry and Physiology·N W Cornell, C A Villee
Jun 15, 1992·Gene·P C WuK E Kendrick
May 1, 1974·Applied Microbiology·H L WangC W Hesseltine
Apr 1, 1969·Journal of Dairy Science·G A Somkuti, F J Babel

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