Release of proteins and enzymes from vesicular compartments by alternating magnetic fields

Physical Chemistry Chemical Physics : PCCP
Andrew BoothSimon J Webb

Abstract

The magnetic release of catalytically active enzymes from vesicular compartments within aggregated nanomaterials has been demonstrated. These nanomaterials, magnetic nanoparticle-vesicle aggregates (MNPVs), were formed by the self-assembly of biotinylated silica-coated Fe3O4 nanoparticles, biotinylated vesicles and tetrameric avidin. The unique features of nanoscale magnetite allow adhesion between membranes to be combined with magnetically triggered transit of reagents across membranes. Adding short spacers between the adhesive biotin groups and the nanoparticle or vesicle surfaces was found to strengthen binding to avidin, with binding of avidin to biotinylated bilayers and biotinylated nanoparticles monitored by quartz crystal microgravimetry with dissipation (QCM-D). Three different reagents were released from the vesicle compartments of MNPVs by a pulse of alternating magnetic field, with the release of a dye modelling the release of small molecule substrates, and the release of cytochrome c modelling the release of biological polymers, such as enzymes. To confirm that enzymes could be released and maintain activity, trypsin was encapsulated and shown to digest casein after magnetically triggered release.

References

Dec 2, 1976·Biochimica Et Biophysica Acta·B Solomon, I R Miller
Mar 28, 1972·Biochemistry·J Babul, E Stellwagen
Jun 17, 1994·Science·S ChiruvoluJ A Zasadzinski
May 29, 1999·Bioconjugate Chemistry·C A LackeyP S Stayton
Feb 24, 2001·Nature·A KarlssonO Orwar
Sep 29, 2001·Biomolecular Engineering·P Walde, S Ichikawa
Feb 6, 2002·Chembiochem : a European Journal of Chemical Biology·C M PaleosD Tsiourvas
May 11, 2002·BMC Biotechnology·Jacques-Philippe ColletierDidier Fournier
Mar 27, 2003·Journal of the American Chemical Society·Chiaki Yoshina-Ishii, Steven G Boxer
Jan 8, 2004·Journal of the American Chemical Society·Shouheng SunGuanxiong Li
Mar 23, 2004·Biochimica Et Biophysica Acta·Gary S GoldbergPeter R Brink
Jul 13, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Ian J Bruce, Tapas Sen
Oct 8, 2005·Organic & Biomolecular Chemistry·Simon J WebbXi Wang
Feb 14, 2006·The Journal of Physical Chemistry. B·Isaac KohDouglas S English
Nov 9, 2006·Journal of the American Chemical Society·Robert J MartSimon J Webb
Sep 18, 2007·Journal of the American Chemical Society·Kwan Ping LiemSimon J Webb
Jun 24, 2008·Nature Reviews. Molecular Cell Biology·Yong-Ling P OwTak W Mak
Jan 22, 2009·Journal of the American Chemical Society·Kim S HorgerMichael Mayer
Apr 24, 2009·Pharmaceutical Research·Robert J MartSimon J Webb
Aug 18, 2009·Journal of Visualized Experiments : JoVE·Carrie Cupp-Enyard
Feb 26, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Bettina van LengerichSteven G Boxer
Apr 10, 2010·Chemical Communications : Chem Comm·Eugene MahonMihail Barboiu
May 21, 2011·Journal of Colloid and Interface Science·Francesco GaleottiAlberto Bolognesi
Aug 18, 2012·Journal of Molecular Recognition : JMR·Robert E Speight, Matthew A Cooper
Mar 6, 2013·Chemical Communications : Chem Comm·Eugene MahonMihail Barboiu
Apr 6, 2013·Science·Gabriel VillarHagan Bayley
May 21, 2013·Accounts of Chemical Research·Simon J Webb
Jul 25, 2013·Accounts of Chemical Research·Mingming Ma, Dennis Bong
Aug 1, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Oliver RolingBart Jan Ravoo
Nov 30, 2013·Chemical Communications : Chem Comm·Zineb MoulineMihail Barboiu
Jan 11, 2014·Chemical Communications : Chem Comm·Néstor López MoraAlexander Kros
Jan 28, 2014·Advances in Colloid and Interface Science·Paul A Beales, T Kyle Vanderlick
Feb 20, 2014·Colloids and Surfaces. B, Biointerfaces·Yanjing ChenGeoffrey D Bothun
Oct 24, 2014·Chemical Communications : Chem Comm·Dhruv ThakarRalf P Richter

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Citations

Aug 30, 2018·Organic & Biomolecular Chemistry·Anna D PetersSimon J Webb
Jul 12, 2019·Blood Advances·Catriona McCallionJohn Burthem

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