PMID: 9180159May 29, 1997Paper

Release of replication-deficient retroviruses from a packaging cell line: interaction with glioma tumor spheroids in vitro

International Journal of Cancer. Journal International Du Cancer
F ThorsenR Bjerkvig

Abstract

The present study describes how various growth conditions affect gene expression and virus production from a retroviral packaging cell line (Liz 9), grown as monolayers and as multicellular spheroids. In addition, to study the direct interaction between packaging cells and tumor tissue of glioma origin, Liz 9 spheroids were confronted with tumor spheroids derived from a human glioma cell line, GaMg. The results show a progressive gene transfer into the tumor tissue, with 9% transfection efficacy after 5 days of co-culture. In comparison, no gene transfer was observed when the Liz 9 spheroids were confronted with normal brain-cell aggregates. The Liz 9 spheroids established from early-passage cultures (passages 7-14) showed limited growth during 28 days, whereas those initiated from late-passage monolayer cultures (passages 39-49) showed extensive growth. Flow-cytometric DNA profiles of monolayers and of spheroids indicated no difference in cell-cycle distribution or ploidy between early and late passages. A cell-viability assay using scanning confocal microscopy revealed mostly viable cells in the Liz 9 spheroids, with only a few dead cells scattered within the structures. The lacZ-gene expression was maintained in early- and i...Continue Reading

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Citations

Oct 14, 2014·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Robert F Shearer, Darren N Saunders
Jan 17, 2015·Journal of Biotechnology·Delyan P IvanovMartin C Garnett
Aug 9, 2016·Journal of Biotechnology·Delyan P IvanovAnna M Grabowska
Nov 26, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·P E OyvindM Lund-Johansen

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