Release of slow-reacting substance of anaphylaxis and leukotriene C4-like immunoreactivity from guinea pig colonic tissue

Prostaglandins
R H WölblingB M Peskar

Abstract

Colonic mucosa, muscularis propria and subserosa from ovalbumin-sensitized guinea pigs were incubated and challenged with antigen in vitro. Slow-reacting substance of anaphylaxis (SRS-A) was determined biologically as well as radioimmunologically in terms of leukotriene (LT) C4-like immunoreactivity. Before antigenic challenge release of immunoreactive LTC4 by all tissues was below or close to the detection limit of the radioimmunoassay. After addition of antigen colonic mucosa released considerable amounts of LTC4-like immunoreactivity, while muscularis propria and subserosa were less active. The biological activity of the SRS-A formed after challenge was antagonized by FPL 55712. Contrary to LTC4-like immunoreactivity release of 6-keto-prostaglandin (PG) F1 alpha was predominant in the subserosa and smaller amounts were released from the smooth muscular and mucosal layers. Synthesis of SRS-A and LTC4-like immunoreactivity, respectively, as well as synthesis of 6-keto-PGF1 alpha was inhibited by the dual inhibitor of lipoxygenase and cyclooxygenase BW755c. The results suggest a role for LTs as local mediators of inflammatory reactions in colonic disease states, particularly those with possible involvement of immunological proc...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R C MurphyB Samuelsson
Oct 1, 1979·The Journal of Pharmacy and Pharmacology·E Beubler, H Juan
Jun 15, 1979·Biochemical Pharmacology·G A HiggsJ R Vane
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P Borgeat, B Samuelsson
Dec 1, 1977·British Journal of Pharmacology·A BennettH L Stockley
Oct 17, 1973·Nature: New Biology·J AugsteinM L Tattersall
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R A LewisE J Corey
Feb 1, 1982·The Journal of Experimental Medicine·A JörgS J Klebanoff
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S E DahlénB Samuelsson
Nov 1, 1982·British Journal of Pharmacology·A C PeatfieldP S Richardson

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Citations

Sep 12, 1986·Biochimica Et Biophysica Acta·K W DreylingB M Peskar
Feb 1, 1986·Prostaglandins·B M PeskarB A Peskar
Nov 1, 1984·The Journal of Clinical Investigation·F R DeRubertisR Saito
Jun 1, 1997·The Journal of Surgical Research·E A BachaH J Krämling
Apr 1, 1988·Scandinavian Journal of Gastroenterology·S T NielsenC B Buonato
Oct 1, 1985·British Journal of Pharmacology·N K Boughton-Smith, B J Whittle

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Anaphylaxis

Anaphylaxis is a serious allergic reaction that is rapid in onset and may cause death.