Release of soluble CD40 ligand after platelet activation: studies on the solubilization phase

Thrombosis Research
Kari OtterdalN O Solum

Abstract

sCD40L is released from platelets as a soluble, proteolyzed form of CD40 ligand (CD40L; CD154) which is exposed on the surface after platelet activation. Ethylenediaminetetraacetate (EDTA), the CD40-blocking antibody G28-5, and GPIIb-IIIa antagonists are known to inhibit the solubilization when added prior to activation. It is assumed that the surface expression of CD40L is a result of a separate fast process and that the solubilization is secondary to this. The release of sCD40L in this solubilization phase has been studied; that is, inhibitory substances were added to platelet-rich plasma (PRP) 10 min after addition of the activation agonist (100 microM SFLLRN), at which time the secretion phase was over as tested with beta-thromboglobulin (beta-TG). G28-5 (10 microg/ml) and EDTA (5 mM) inhibited the solubilization phase which did not require the presence of an activation agonist. Prostaglandin E1 (PGE1; 20 microM) and cytochalasin D (C8273; 60 and 100 microM), which exert their effects intracellularly, inhibited the solubilization even in the presence of abciximab (ReoPro; 40 microg/ml). The intracellular effect was not related to CD40L-containing microparticles as demonstrated by ultracentrifugation. Intracellular alkaliniz...Continue Reading

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Citations

Jul 7, 2009·Archivum Immunologiae Et Therapiae Experimentalis·Jennifer M SowaBennett D Elzey
Apr 8, 2010·Journal of Thrombosis and Thrombolysis·Peter S MunkAlf I Larsen
Mar 13, 2015·Experimental Hematology & Oncology·Antoine DewitteJean Ripoche
Nov 29, 2007·Internal and Emergency Medicine·F SantilliG Davì
Jun 15, 2007·Critical Reviews in Biochemistry and Molecular Biology·Bénédicte CauweGhislain Opdenakker
Oct 4, 2005·Médecine sciences : M/S·Yahsou DelmasJean Ripoche
Oct 27, 2007·American Journal of Respiratory and Critical Care Medicine·Kari OtterdalJan K Damås

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