PMID: 7370738Apr 14, 1980Paper

Release of synaptosomal dopamine formed from tyrosine and L-DOPA

Brain Research
S P BagchiT M Smith

Abstract

The present report compares the synaptosomal release of [3H]dopamine, continuously forming from [3H]DOPA, with that of [14C]dopamine, forming from [14C]tyrosine as a marker of dopaminergic nerve endings. For the purpose of the comparison, synaptosomal (P2) preparations from rat caudate nuclei were incubated with L-[3H]DOPA and [14C]tyrosine for 10 min and the particulates were rapidly separated from the medium postincubation. The separated fractions were analyzed for the level of double labelled (14C/3H) dopamine and the synaptosomal content of the labelled substrates. Of the total labelled dopamine formed, the fraction that was present in the medium, following the synaptosomal release, was determined. Tested were the release enhancing effects of various additions which included several known dopaminergic agents, serotonin and 5-hydroxytryptophan. The data show that the addition of dopamine to the incubation mixture to either 0.5 or 1.0 muM concentration markedly enhanced the release of labelled dopamine. Serotonin when added to 5.0 muM concentration also raised the medium content of labelled dopamine but it was ineffective at 1.0 muM. 5-Hydroxytryptophan, 1.0 or 5.0 muM, had no effect. For the comparison of the release enhanci...Continue Reading

References

Nov 1, 1976·The Journal of Pharmacy and Pharmacology·P A Shore
Jun 1, 1977·Journal of Neurochemistry·G Sperk, R J Baldessarini
Jan 1, 1975·Journal of Neural Transmission·M LindqvistA Carlsson
Mar 25, 1975·Naunyn-Schmiedeberg's Archives of Pharmacology·A PhilippuH Lentzen
Jun 1, 1973·Biochemical Pharmacology·S P Bagchi, E P Zarycki
Jul 1, 1974·Journal of Neurochemistry·R L Patrick, J D Barchas
Sep 1, 1969·Journal of Neurochemistry·S FahnL J Côté
Oct 2, 1970·Science·K Y NgI J Kopin

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