Release of the angiogenic cytokine vascular endothelial growth factor (VEGF) from platelets: significance for VEGF measurements and cancer biology

British Journal of Cancer
R E BanksP J Selby

Abstract

Vascular endothelial growth factor (VEGF) is a potent angiogenic factor with a key role in several pathological processes, including tumour vascularization. Our preliminary observations indicated higher VEGF concentrations in serum samples than in matched plasma samples. We have now demonstrated that this difference is due to the presence of VEGF within platelets and its release upon their activation during coagulation. In eight healthy volunteers, serum VEGF concentrations ranged from 76 to 854 pg ml(-1) and were significantly higher (P < 0.01) than the matched citrated plasma VEGF concentrations, which ranged from < 9 to 42 pg ml(-1). Using platelet-rich plasma, mean (s.d.) platelet VEGF contents of 0.56 (0.36) pg of VEGF 10(-6) platelets were found. Immunocytochemistry demonstrated the cytoplasmic presence of VEGF within megakaryocytes and other cell types within the bone marrow. From examination of the effects of blood sample processing on circulating VEGF concentrations, it is apparent that for accurate measurements, citrated plasma processed within 1 h of venepuncture should be used. Serum is completely unsuitable. The presence of VEGF within platelets has implications for processes involving platelet and endothelial cell...Continue Reading

Citations

Jul 9, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·A WeltermannP A Kyrle
Jul 11, 2000·British Journal of Haematology·K L Talks, A L Harris
Dec 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E TaniguchiM Sata
Nov 18, 2000·Arthritis and Rheumatism·R MeliconiA Facchini
Dec 21, 2000·Current Oncology Reports·D A Hazelton, T C Hamilton
Jul 5, 2001·Diabetic Medicine : a Journal of the British Diabetic Association·N ChaturvediUNKNOWN EUCLID Study Group
Jan 25, 2002·Blood·Martin B SteinsRolf M Mesters
Nov 28, 2002·British Journal of Cancer·R SalgadoL Y Dirix
Apr 20, 2004·European Journal of Haematology·Maria-Christina KyrtsonisGerassimos A Pangalis
May 4, 2004·Annals of Medicine·Gregory Y H Lip, Andrew D Blann
Oct 8, 2005·Angiogenesis·Charlotta Dabrosin
Oct 13, 2006·Clinical and Experimental Hypertension : CHE·Timoleon VyzantiadisChrysanthos Zamboulis
Jun 12, 2008·Tissue Engineering. Part C, Methods·Isabelle CatelasSam Helgerson
Nov 13, 2008·Journal of Cardiovascular Medicine·Ugo TestaGian Luigi Condorelli
Jun 17, 2014·Oncology Letters·Ahmed AbdallahRabab Gaafar
Oct 1, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Brian A KellyMartha M Murray
Mar 25, 2016·Biomaterials Science·David G BelairWilliam L Murphy
Jul 7, 2017·Cancer Metastasis Reviews·Marek Z WojtukiewiczKenneth V Honn
Jul 4, 2018·European Archives of Psychiatry and Clinical Neuroscience·Karen M Ryan, Declan M McLoughlin
Jan 4, 2020·Aesthetic Plastic Surgery·Antonio Gustavo ZamparDimas T Covas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.