Release of the antioxidants ascorbate and urate from a nitrergically-innervated smooth muscle

British Journal of Pharmacology
E Lilley, A Gibson

Abstract

1. The main object of the present study was to determine whether ascorbate, an antioxidant which has been shown to protect nitric oxide (NO) from attack by scavenger molecules, might be released from nitrergically-innervated smooth muscle; ascorbate release from the rat anococcygeus was measured by use of h.p.l.c. with electrochemical detection. 2. Incubation of rat anococcygeus muscles in normal physiological salt solution (PSS; 30 min) resulted in release of ascorbate into the bathing medium (7.7 +/- 0.9 nmol g-1 tissue). This release was increased by 96% when muscles were incubated in high K+ (70 mM) PSS. The resting release of ascorbate was unaffected by tetrodotoxin (TTX; 1 microM), omega-conotoxin GVIA (10 nM) or omission of calcium ions from the PSS (with addition of 0.2 mM EGTA), but all three procedures attenuated the increased release observed under depolarizing conditions. Resting release of ascorbate was unaffected by glutamate (100 microM), aspartate (100 microM), gamma-aminobutyric acid (100 microM) or carbachol (50 microM). 3. A second h.p.l.c. peak, which always preceded the ascorbate peak, was identified as urate. Urate release from the anococcygeus, following 30 min incubation in normal PSS, was 64.6 +/- 12.7 ...Continue Reading

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Citations

Oct 7, 1998·European Journal of Pharmacology·A EllisM J Rand
Dec 16, 1998·European Journal of Pharmacology·C GöçmenF Baysal
Mar 22, 2000·British Journal of Pharmacology·E E Colpaert, R A Lefebvre
May 12, 2000·British Journal of Pharmacology·E E Colpaert, R A Lefebvre
Feb 28, 2002·British Journal of Pharmacology·E E ColpaertR A Lefebvre
Mar 6, 2002·British Journal of Pharmacology·Juan V Esplugues
Dec 7, 2005·British Journal of Pharmacology·Csaba Kónya, Péter Ferdinandy
Aug 24, 1999·The Journal of Surgical Research·J J CullenL W Oberley
Apr 18, 2012·Anatomia, histologia, embryologia·G Scala, L Maruccio

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