PMID: 191091Mar 29, 1977Paper

Release of the phosphodiesterase activator by cyclic AMP-dependent ATP:protein phosphotransferase from subcellular fractions of rat brain

Biochimica Et Biophysica Acta
M E GnegyP Uzunov

Abstract

The subcellular distribution of the endogenous phosphodiesterase activator and its release from membranes by a cyclic AMP-dependent ATP:protein phosphotransferase was studied in fractions and subfractions of rat brain homogenate. These fractions were obtained by differential centrifugation and sucrose density gradient; their identity was ascertained by electron microscopy and specific enzyme markers. In the subcellular particulate fractions, the concentration of activator is highest in the microsomal fraction, followed by the mitochondrial and nuclear fractions. Gradient centrifugation of the main mitochondrial subfraction revealed that activator was concentrated in those fractions containing mainly synaptic membranes. Activator was releasted from membranes by a cyclic AMP-dependent phosphorylation of membrane protein. The release of activator occurred mainly from the mitochondrial subfractions containing synaptic membranes and synaptic vesicles. The data support the view that a release of activator from membranes may be important in normalizing the elevated concentration of cyclic AMP following persistent transsynaptic activation of adenylate cyclase.

References

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Citations

Jan 1, 1982·Diabetologia·S TomlinsonB L Brown
Feb 1, 1983·Neurochemical Research·W Hoss, M Formaniak
Jun 1, 1980·Neurochemical Research·F Vargas, A Guidotti
Apr 1, 1985·Brain Research·M A Wilson, R Gillette
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M MemoI Hanbauer
Jun 14, 1978·Biochemical and Biophysical Research Communications·T E Donnelly
Jul 1, 1980·Pharmacological Research Communications·A Lucchelli
Oct 30, 1978·Biochemical and Biophysical Research Communications·A VandermeersJ Christophe
Sep 27, 1983·Biochemistry·P A Bartlett, C K Marlowe
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Jan 1, 1980·CRC Critical Reviews in Biochemistry·R H Kretsinger
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Jan 1, 1980·Annals of the New York Academy of Sciences·I HanbauerH Y Yang
Jan 1, 1980·Annals of the New York Academy of Sciences·B WeissT L Wallace
Sep 1, 1983·American Journal of Hematology·J A WarthK Near

Related Concepts

Metazoa
Brain
Enzyme Activation
Tissue Membrane
Osmosis
Phosphoric diester hydrolase
Protein KINASE
Subcellular Fractions
Synaptic Membranes
Synaptic Vesicles

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