Releasing iron from ferritin protein nanocage by reductive method: The role of electron transfer mediator

Biochimica Et Biophysica Acta. General Subjects
Prashanth Kumar KoochanaRabindra K Behera

Abstract

Ferritin detoxifies excess of free Fe(II) and concentrates it in the form of ferrihydrite (Fe2O3·xH2O) mineral. When in need, ferritin iron is released for cellular metabolic activities. However, the low solubility of Fe(III) at neutral pH, its encapsulation by stable protein nanocage and presence of dissolved O2 limits in vitro ferritin iron release. Physiological reducing agent, NADH (E1/2 = -330 mV) was inefficient in releasing the ferritin iron (E1/2 = +183 mV), when used alone. Thus, current work investigates the role of low concentration (5-50 μM) of phenazine based electron transfer (ET) mediators such as FMN, PYO - a redox active virulence factor secreted by Pseudomonas aeruginosa and PMS towards iron mobilization from recombinant frog M ferritin. The presence of dissolved O2, resulting in initial lag phase and low iron release in FMN, had little impact in case of PMS and PYO, reflecting their better ET relay ability that facilitates iron mobilization. The molecular modeling as well as fluorescence studies provided further structural insight towards interaction of redox mediators on ferritin surface for electron relay. Reductive mobilization of iron from ferritin is dependent on the relative rate of NADH oxidation, diss...Continue Reading

Citations

Feb 20, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Prashanth Kumar KoochanaRabindra K Behera
Jan 11, 2020·Frontiers in Neuroscience·Barry B Muhoberac, Ruben Vidal
Feb 19, 2021·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Prashanth Kumar KoochanaRabindra K Behera
Apr 22, 2021·Metallomics : Integrated Biometal Science·Marina PlaysRaphaël Rodriguez
Oct 28, 2019·The Journal of Medical Investigation : JMI·Kazumi Takaishi, Hiroshi Kitahata

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