Relevance of allosteric conformations and homocarnosine concentration on carnosinase activity

Amino Acids
Verena PetersBenito Yard

Abstract

Activity of carnosinase (CN1), the only dipeptidase with substrate specificity for carnosine or homocarnosine, varies greatly between individuals but increases clearly and significantly with age. Surprisingly, the lower CN1 activity in children is not reflected by differences in CN1 protein concentrations. CN1 is present in different allosteric conformations in children and adults since all sera obtained from children but not from adults were positive in ELISA and addition of DTT to the latter sera increased OD450 values. There was no quantitative difference in the amount of monomeric CN1 between children and adults. Further, CN1 activity was dose dependently inhibited by homocarnosine. Addition of 80 microM homocarnosine lowered V (max) for carnosine from 440 to 356 pmol/min/microg and increased K (m) from 175 to 210 microM. The estimated K (i) for homocarnosine was higher (240 microM). Homocarnosine inhibits carnosine degradation and high homocarnosine concentrations in cerebrospinal fluid (CSF) may explain the lower carnosine degradation in CSF compared to serum. Because CN1 is implicated in the susceptibility for diabetic nephropathy (DN), our findings may have clinical implications for the treatment of diabetic patients wi...Continue Reading

References

Oct 11, 1979·Biochimica Et Biophysica Acta·F L MargolisG J Elgar
Mar 1, 1975·Journal of Neurochemistry·T L PerryJ Kennedy
Jun 15, 1985·The Biochemical Journal·J F LenneyR P George
May 1, 1970·Comparative Biochemistry and Physiology·K G Crush
Oct 1, 1968·Journal of Neurochemistry·T L PerryD Love
Aug 18, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·J F LenneyG S Rinzler
Feb 1, 1994·Clinica Chimica Acta; International Journal of Clinical Chemistry·W S WassifT J Peters
Jun 1, 1994·Experimental Cell Research·G A McFarland, R Holliday
Aug 1, 1993·The International Journal of Biochemistry·A A Boldyrev
Sep 23, 1998·The International Journal of Biochemistry & Cell Biology·A R Hipkiss
Jan 1, 1992·Molecular Aspects of Medicine·P J QuinnV E Formazuyk
Apr 3, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·A PegovaA Boldyrev
May 7, 2002·Journal of Neuroscience Research·Rinat TabakmanRon Kohen
Oct 31, 2002·Equine Veterinary Journal. Supplement·M DunnettP A Harris
Dec 11, 2002·The Journal of Biological Chemistry·Michael TeufelTatiana Smirnova
Nov 29, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Erwin E W JansenNanda M Verhoeven
Nov 7, 2007·BMC Neurology·Cynthia M BalionAfisi S Ismaila
Jan 10, 2009·Journal of Applied Physiology·Audrey BaguetWim Derave

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Citations

Sep 23, 2014·Regulatory Peptides·V PetersH Köppel
Jul 25, 2015·Amino Acids·Verena PetersEmile de Heer
Jun 18, 2015·Amino Acids·Verena PetersClaus P Schmitt
Apr 15, 2015·The Journal of Endocrinology·Elisabete A ForsbergSergiu Bogdan Catrina
Oct 14, 2017·Journal of Inherited Metabolic Disease·Verena PetersClaus P Schmitt
Aug 5, 2017·Journal of Enzyme Inhibition and Medicinal Chemistry·Verena PetersGiulio Vistoli
Apr 13, 2012·American Journal of Physiology. Renal Physiology·Inge EveraertWim Derave
Oct 19, 2013·Physiological Reviews·Alexander A BoldyrevWim Derave
Sep 22, 2015·American Journal of Physiology. Endocrinology and Metabolism·Sanne StegenWim Derave
Jul 17, 2019·Current Medicinal Chemistry·Ken-Ichiro Tanaka, Masahiro Kawahara
Mar 28, 2019·Current Medicinal Chemistry·Verena PetersClaus Peter Schmitt

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