Abstract
Although astrocytes express gamma-aminobutyric acid subtype-A (GABAA) receptors in the mature brain, GABAA receptor expression in a cultivation state remains controversial. In this study, we investigated the alteration of astrocytic GABAA receptor expression in in vitro and in vivo studies to elucidate the relevance of astrocytic activation to reductions of astrocytic GABAA receptors. The GABA-evoked Cl- current (GABAA response) in cultured astrocytes was determined by recording in the whole-cell mode using a conventional patch-clamp technique under voltage-clamp conditions. The respective amplitudes of GABAA responses on days in vitro 1, 3-5, 7-10, and 12-15 were 1019+/-97, 512+/-76, 84+/-21, and 22+/-9 pA, respectively, suggesting that the GABAA response subsequently diminished with in vitro aging. In immunohistochemical and biochemical analyses, the expression of GABAA receptor beta-subunit decreased, whereas expressions of glial fibrillary acidic protein (GFAP) and S100B, hallmarks of astrocytic activation, increased dramatically in the cultured astrocytes with in vitro aging. With the use of [3H]SR95531, a GABAA-specific ligand, at 24 h after transient focal ischemia, binding was significantly reduced in the astrocytic fra...Continue Reading
References
Jul 31, 1992·Science·S J MossR L Huganir
Mar 1, 1991·Neurochemical Research·G H HansenL Hösli
Oct 1, 1991·Stroke; a Journal of Cerebral Circulation·A AurellK G Haglid
Jan 1, 1990·Experimental Brain Research·E Hösli, L Hösli
Sep 18, 1990·Neuroscience Letters·L HösliH Schramek
Jan 1, 1990·Experimental Brain Research·E Hösli, L Hösli
Jan 1, 1990·Neurology·A Delacourte
Aug 14, 1989·Neuroscience Letters·K MuraseM Randic
Sep 1, 1987·Stroke; a Journal of Cerebral Circulation·L PerssonS Påhlman
May 1, 1986·Stroke; a Journal of Cerebral Circulation·J B BedersonH Bartkowski
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D Kligman, D R Marshak
Oct 1, 1993·Hippocampus·H LiR D Schwartz
Jan 1, 1997·Stroke; a Journal of Cerebral Circulation·J NakagawaraN A Lassen
Sep 26, 1997·Stroke; a Journal of Cerebral Circulation·Y DongY Yonekura
Oct 28, 1997·Stroke; a Journal of Cerebral Circulation·U MisslerM Kaps
Oct 28, 1997·Stroke; a Journal of Cerebral Circulation·T BüttnerW Kuhn
May 29, 1998·Brain Research. Molecular Brain Research·K MizukamiD M Armstrong
Apr 6, 1999·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·N TateishiK Kondo
Jun 25, 1999·Cell·B A Barres
Jun 25, 1999·Cell·F DoetschA Alvarez-Buylla
Feb 7, 2001·Science·E M UllianB A Barres
Apr 12, 2001·Journal of Neurochemistry·R D Schwartz-Bloom, R Sah
Jun 19, 2001·The Journal of Physiology·K NäglerF W Pfrieger
Nov 10, 2001·Science·D H MauchF W Pfrieger
May 3, 2002·Nature·Hongjun SongFred H Gage
Jun 25, 2002·Trends in Neurosciences·Gilles BonventoLuc Pellerin
Dec 21, 2002·Nature Neuroscience·Rheinallt Parri, Vincenzo Crunelli
Mar 20, 2003·Microscopy Research and Technique·Matthias RothermundtVolker Arolt
Oct 3, 2003·Trends in Neurosciences·Michal Slezak, Frank W Pfrieger
May 18, 2004·Neurochemistry International·Gerald A Dienel, Nancy F Cruz
Jul 6, 2004·Annals of Neurology·Mark S WainwrightLinda J Van Eldik
Aug 26, 2004·Brain Research·Yuko YasudaSetsuya Fujita
Sep 1, 2004·Biochemical and Biophysical Research Communications·Ingo MarenholzGünter Fritz
Apr 29, 2005·Current Drug Targets. CNS and Neurological Disorders·T AsanoN Tateishi
Citations
Aug 27, 2009·Cellular and Molecular Neurobiology·Iva PrajerovaMiroslava Anderova
Jun 21, 2011·Journal of Neuroinflammation·Kumiko TanabeHiroki Iida
May 9, 2012·PloS One·Laurence DesruesHélène Castel
Mar 29, 2011·Journal of Neuroscience Methods·Annalisa BernareggiFabrizio Eusebi
Jul 18, 2014·Archives of Pharmacal Research·Hyun Myung KoChan Young Shin
Aug 29, 2018·British Journal of Pharmacology·Kouji Fukuyama, Motohiro Okada
Nov 6, 2018·Neurochemical Research·Adriana Fernanda K VizueteCarlos-Alberto Gonçalves
Jan 12, 2019·Pharmacology Research & Perspectives·Motohiro OkadaYuto Ueda
Aug 2, 2019·International Journal of Molecular Sciences·Motohiro OkadaYuto Ueda
May 22, 2019·Biomolecules·Tomosuke NakanoMotohiro Okada
Feb 21, 2019·Pharmacology Research & Perspectives·Motohiro OkadaYuto Ueda
Mar 12, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Motohiro OkadaYuto Ueda