[Relevance of cell culture models in cutaneous tumour biology: part II: complex culture systems].

Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete
J Hatina, T Ruzicka

Abstract

There are several limitations to the use of the classical monolayer cell culture and the results obtained by means of it. The two-dimensional architecture and the analysis of pure cell populations of individual cell lines are the most several deviations from the situation prevailing in tissues in vivo, with inevitable consequences for the phenotypic traits displayed on the one hand, and for the genome structure and expression on the other hand. Newer developments in cell culture methodology seek approaches to mimic the in vivo situation in the cell culture as closely as possible. Remarkable variety of such approaches can be noticed, ranging from relative simple three-dimensional conditions of culturing pure cell lines on collagen gels or in form of multicell tumor spheroids. More complex forms try to combine multiple cell types in a single co-culture, e.g. of tumour cells and stromal fibroblasts. The most complex and most revealing among the three-dimensional culture arrangements is unquestionably the organotypic skin culture, in which all the relevant skin cell types are combined in a tissue-resembling construct, with resulting marked similarity to the anatomical structure of normal human skin. Several crucial results were obt...Continue Reading

References

Jan 1, 1992·Pharmacology & Therapeutics·J DiGiovanni
Jul 8, 1999·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·M T Santini, G Rainaldi
Aug 10, 1999·Nature·W C HahnR A Weinberg
Sep 1, 1999·The Journal of Investigative Dermatology·M VaccarielloJ A Garlick
Jan 7, 2000·The American Journal of Pathology·F MeierM Herlyn
Oct 18, 2000·Critical Reviews in Oncology/hematology·B Desoize, J Jardillier
May 2, 2002·The Journal of Investigative Dermatology·Anthony J DickerNicholas A Saunders
Oct 3, 2002·Nature Medicine·Mirella LazarovPaul A Khavari
Dec 12, 2002·Skin Pharmacology and Applied Skin Physiology·C Faller, M Bracher
Jun 5, 2003·Nature Reviews. Cancer·Mary J C HendrixRichard E B Seftor
Jun 20, 2003·Annals of the New York Academy of Sciences·Mary J C HendrixRichard E B Seftor
Jul 4, 2003·The Journal of Investigative Dermatology·Adarsh V MudgilJonathan A Garlick
Jul 4, 2003·The Journal of Investigative Dermatology·Douglas E Brash
Dec 4, 2003·International Journal of Cancer. Journal International Du Cancer·Frank AndrianiJonathan A Garlick
Jan 8, 2004·The Journal of Investigative Dermatology·Alexander MargulisJonathan A Garlick
Oct 14, 2004·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·T Hunziker
Nov 2, 2004·Nature Reviews. Cancer·Margareta M Mueller, Norbert E Fusenig
Apr 28, 2005·International Journal of Cancer. Journal International Du Cancer·Weitian ZhangJonathan A Garlick
Jun 14, 2005·Nature Genetics·Yakov ChudnovskyPaul A Khavari
Jul 27, 2005·Seminars in Cancer Biology·Keiran S M SmalleyMeenhard Herlyn
Oct 19, 2005·Cancer Research·Dong FangMeenhard Herlyn
Nov 17, 2005·Cancer Research·Elisabeth A SeftorMary J C Hendrix
Dec 20, 2005·The Journal of Investigative Dermatology. Symposium Proceedings·Nikolas K Haass, Meenhard Herlyn
Mar 28, 2006·Journal of Cellular and Molecular Medicine·W A Schulz, J Hatina
Mar 28, 2006·Journal of Cellular and Molecular Medicine·Elisabeth A SeftorMary J C Hendrix
Feb 27, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Elena MonzaniCaterina A M La Porta
Nov 28, 2007·Annual Review of Pathology·Ryan J Ward, Peter B Dirks

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Related Papers

Archives of Toxicology. Supplement. = Archiv Für Toxikologie. Supplement
A M LaaksonenO O Hänninen
Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer
R M Sutherland, R E Durand
© 2021 Meta ULC. All rights reserved