PMID: 11905985Mar 22, 2002Paper

Relevance of different striatal markers in assessment of the MPP+-induced dopaminergic nigrostriatal injury in rat

Journal of Neurochemistry
Stéphanie BarcSylvie Chalon

Abstract

Many striatal dopaminergic markers are available for estimating the degree of the nigrostriatal lesion by MPTP/MPP+, but the changes of these markers are not perfectly matched. In this study we investigated different striatal markers and determined which ones closely reflected the nigrostriatal alteration. The in vivo binding of (E)-N-(3-iodoprop-2-enyl)-2-beta-carbomethoxy-3beta-(4'-methylphenyl)nortropane (PE2I), a selective and potent inhibitor of the neuronal dopamine transporter (DAT) was considered as the reference index of injury of striatal dopaminergic nerve-endings. Rats received a 10-microg MPP+ injection in the right substantia nigra and were killed at 7 days after lesion. The results were as follows: (i) a decrease (66%) of the biodistribution of [125I]PE2I; (ii) a great reduction of the DAT expression measured by the binding of [125I]PE2I in striatal membranes (Bmax decreased by 54%) and in cerebral slices (88%); (iii) an 80% inhibition of the vesicular monoamine transporter expression revealed by the binding of [3H]dihydrotetrabenazine in cerebral slices; (iv) a robust decrease in the quantity of DA and its metabolites (about 50-60%); (v) a slight modification of the DAT activity with a decreased number of functi...Continue Reading

Citations

Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michelle SmeyneRichard Jay Smeyne
Jul 21, 2009·Neurobiology of Disease·Afsaneh GaillardMohamed Jaber
May 17, 2008·CNS Neuroscience & Therapeutics·Patrick EmondSylvie Chalon
Mar 26, 2005·Brain Research. Molecular Brain Research·Ciaran J FahertyRichard J Smeyne
Mar 11, 2008·Biochemical Pharmacology·Gang ZhaoLi-He Guo
Oct 23, 2012·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Stéphanie PainSylvie Chalon
Feb 21, 2018·Neurotoxicity Research·Natalia Moreno-GalarzaJosé Aguilera

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