Relevance of in vitro agar based screens to characterize the anti-fungal activities of bacterial endophyte communities

BMC Microbiology
H R ShehataManish N Raizada

Abstract

Endophytes are microbes that inhabit internal plant tissues without causing disease. Plant microbial communities consist of large numbers of endophyte species. Understanding the functions of these endophytes is a major challenge. An important function of some endophytes is to suppress fungal pathogens. Typically, plant associated microbes are screened for anti-fungal activities in vitro using the high-throughput dual culture screen, but it is not clear whether this method correlates with the activities of these microbes in planta. Furthermore, it is not clear whether in vitro screening captures all of the microbes that show this activity inside plants. The objective of this study was to evaluate the relevance of the in vitro dual culture method for screening endophytes with anti-fungal activity. In parallel, 190 bacterial endophytes from the corn grass family (Zea) were screened for suppression of two fungal pathogens (Sclerotinia homoeocarpa and Rhizoctonia solani) using the in vitro dual culture method, and in planta using the model plant, creeping bentgrass. All endophytes that showed anti-fungal activity in planta against Sclerotinia homoeocarpa and Rhizoctonia solani (3 or 4 strains, respectively, out of 190), were capture...Continue Reading

References

Jan 13, 2005·Canadian Journal of Microbiology·Franziska FaltinGabriele Berg
Aug 15, 2006·Molecular Plant-microbe Interactions : MPMI·Mónica Rosenblueth, Esperanza Martínez-Romero
Jul 7, 2009·Annual Review of Microbiology·Ben Lugtenberg, Faina Kamilova
May 4, 2011·Current Opinion in Plant Biology·Barbara Reinhold-Hurek, Thomas Hurek
Mar 10, 2015·Applied Microbiology and Biotechnology·Zahoor Ahmed WaniSyed Riyaz-Ul-Hassan

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Citations

Feb 6, 2018·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Amena KhatunTofazzal Islam
Nov 1, 2018·Anais Da Academia Brasileira De Ciências·Ariana A RodriguesJosé Daniel G Vieira
Feb 12, 2017·Foodborne Pathogens and Disease·Hanan R ShehataManish N Raizada
Jun 24, 2018·Archives of Microbiology·Adetomiwa Ayodele Adeniji, Olubukola Oluranti Babalola

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