PMID: 9543015May 16, 1998Paper

Relevance of kinetochore size and microtubule-binding capacity for stable chromosome attachment during mitosis in PtK1 cells

Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology
B F McEwenA B Heagle

Abstract

Chromosomes attach to the mitotic spindle via their kinetochores. The average number of spindle microtubules binding to each kinetochore varies with species, the stage of mitosis, and the length of time that the kinetochore has been attached to the spindle. In this report, we investigate how kinetochore microtubule number varies with kinetochore size and chromosome size in PtK1 cells. From an analysis of serial-section electron micrographs, we determined that the average surface area of metaphase, taxol-treated metaphase, and anaphase kinetochores is 0.16 +/- 0.05 microm2 (N = 181). Surprisingly, kinetochore microtubules are packed more densely on the smaller kinetochores, as seen by a reduction in the average spacing between kinetochore microtubules from 89 nm to 59 nm. Our interpretation of this result is that PtK1 cells require a minimum kinetochore microtubule-binding capacity for survival during repeated rounds of mitotic division. We estimate the lower limit to be 23 kinetochore microtubules and suggest that this capacity is required to ensure stable attachment during the dynamic and highly stochastic process of kinetochore fiber formation. There is a modest but statistically significant increase in kinetochore microtubul...Continue Reading

Citations

Jul 16, 2004·Molecular and Cellular Biology·Gerhard WielandPeter Hemmerich
Apr 26, 2006·The Journal of Cell Biology·Lisa A CameronE D Salmon
May 4, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Han Zhang, R Kelly Dawe
Aug 11, 2015·Nature Cell Biology·Valentin MagidsonAlexey Khodjakov
Nov 4, 2016·Cell Division·L John Gagliardi, Daniel H Shain
Feb 11, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Danielle V IrvineRichard Saffery
Oct 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Richard P Sear, Martin Howard
Feb 20, 2013·The Journal of Cell Biology·J Richard McIntoshEkaterina L Grishchuk
Feb 25, 2014·Cellular and Molecular Bioengineering·Jeremy M Chacón, Melissa K Gardner
Oct 10, 2020·Current Biology : CB·Alexander A KukrejaAjit P Joglekar
May 1, 2018·Current Biology : CB·Danica DrpicHelder Maiato
Aug 11, 2018·Trends in Cell Biology·Mary Williard EltingSophie Dumont
Oct 25, 2012·Journal of Cell Science·Scott C SchuylerVivian Jen-Wei Kuan
Aug 15, 1998·Trends in Cell Biology·C L Rieder, E D Salmon
Oct 8, 2021·Scientific Reports·Klára PlačkováFrantišek Zedek
Nov 13, 2009·Current Biology : CB·Sophie Dumont, Timothy J Mitchison
Dec 15, 2010·Current Biology : CB·Kerry Bloom, Elaine Yeh

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