Relevance of the use of rubber dam in microbiological sampling of carious dentine

Caries Research
E A Kidd, D Beighton

Abstract

The aim of this study was to determine the role of rubber dam isolation and the influence of salivary contamination during cavity preparation on the collection of samples of dentine for microbiological examination. Cavities were prepared in 23 carious teeth. Access to caries was gained and a sample of soft, stained demineralized tissue was taken with a round bur. After removing the rubber dam each cavity was flooded with saliva and after thorough washing and drying a second dentine sample was taken. Cavity preparation was completed and the cavity flooded with saliva. After thorough washing and drying a final sample was taken. A data set from another study served as a control for bacterial contamination at the end of cavity preparation where a rubber dam remained in place throughout. There were no significant differences in the numbers of bacteria in dentine samples gathered with and without rubber dam, which implies that salivary contamination can be removed by thorough washing and drying of the cavity.

Citations

Oct 10, 2003·Australian Critical Care : Official Journal of the Confederation of Australian Critical Care Nurses·Marianne O'Reilly
Sep 20, 2016·IEEE Journal of Selected Topics in Quantum Electronics : a Publication of the IEEE Lasers and Electro-optics Society·Robert C LeeDaniel Fried
Apr 2, 2008·Journal of Dentistry·C H Chu, Edward C M Lo
Apr 15, 2015·The Open Dentistry Journal·Caroline DiasPaula Lambert
Oct 16, 2020·Clinical Oral Investigations·Taciana Marco Ferraz CaneppeleEduardo Bresciani

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