Reliability of maximal mitochondrial oxidative phosphorylation in permeabilized fibers from the vastus lateralis employing high-resolution respirometry

Physiological Reports
Daniele A CardinaleFilip J Larsen

Abstract

The purpose was to assess the impact of various factors on methodological errors associated with measurement of maximal oxidative phosphorylation (OXPHOS) in human skeletal muscle determined by high-resolution respirometry in saponin-permeabilized fibers. Biopsies were collected from 25 men to assess differences in OXPHOS between two muscle bundles and to assess the correlation between OXPHOS and the wet weight of the muscle bundle. Biopsies from left and right thighs of another five subjects were collected on two occasions to compare limbs and time-points. A single muscle specimen was used to assess effects of the anesthetic carbocaine and the influence of technician. The difference in OXPHOS between two fiber-bundles from the same biopsy exhibited a standard error of measurement (SEM) of 10.5 pmol · s-1  · mg-1 and a coefficient of variation (CV) of 15.2%. The differences between left and right thighs and between two different time-points had SEMs of 9.4 and 15.2 pmol · s-1  · mg-1 and CVs of 23.9% and 33.1%, respectively. The average (±SD) values obtained by two technicians monitoring different bundles of fibers from the same biopsy were 31.3 ± 7.1 and 26.3 ± 8.1 pmol · s-1  · mg-1 . The time that elapsed after collection of...Continue Reading

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Dec 30, 2016·Scandinavian Journal of Medicine & Science in Sports·B Ekblom

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Citations

Jun 26, 2018·Current Opinion in Clinical Nutrition and Metabolic Care·Mario OstSonia Moreno-Gomez
Dec 12, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christopher PignanelliJamie F Burr
Sep 4, 2018·Frontiers in Physiology·Daniele A CardinaleRobert Boushel
Jan 11, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Macsue JacquesNir Eynon
Jun 11, 2021·Journal of Applied Physiology·Daniele A CardinaleFilip J Larsen

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Methods Mentioned

BETA
biopsies
biopsy

Software Mentioned

SPSS
Prism
DatLab

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