Reliability of non-lethal surveillance methods for detecting ranavirus infection

Diseases of Aquatic Organisms
Matthew J GrayJason T Hoverman

Abstract

Ranaviruses have been identified as the etiologic agent in many amphibian die-offs across the globe. Polymerase chain reaction (PCR) is commonly used to detect ranavirus infection in amphibian hosts, but the test results may vary between tissue samples obtained by lethal and non-lethal procedures. Testing liver samples for infection is a common lethal sampling technique to estimate ranavirus prevalence because the pathogen often targets this organ and the liver is easy to identify and collect. However, tail clips or swabs may be more practicable for ranavirus surveillance programs compared with collecting and euthanizing animals, especially for uncommon species. Using PCR results from liver samples for comparison, we defined false-positive test results as occurrences when a non-lethal technique indicated positive but the liver sample was negative. Similarly, we defined false-negative test results as occurrences when a non-lethal technique was negative but the liver sample was positive. Using these decision rules, we estimated false-negative and false-positive rates for tail clips and swabs. Our study was conducted in a controlled facility using American bullfrog Lithobates catesbeianus tadpoles; false-positive and false-negativ...Continue Reading

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Citations

Apr 12, 2013·Diseases of Aquatic Organisms·Anke C StöhrRachel E Marschang
Oct 1, 2014·Diseases of Aquatic Organisms·A F Johnson, J L Brunner
Aug 27, 2015·Molecular Ecology Resources·Emily M HallJesse L Brunner
May 7, 2013·The Veterinary Clinics of North America. Exotic Animal Practice·La'Toya V Latney, Eric Klaphake
Mar 29, 2014·PloS One·Roberto BrenesDebra L Miller
Jun 4, 2015·PloS One·Alexandra C NorthAmber G F Griffiths
Apr 21, 2017·Diseases of Aquatic Organisms·Mickey AghaBrian D Todd
Aug 3, 2017·EcoHealth·Steven J A KimbleJason T Hoverman
Jun 20, 2018·Diseases of Aquatic Organisms·Isaac StandishTeresa Lewis
Mar 8, 2018·PloS One·Matthew J GrayDebra L Miller
Aug 9, 2019·Diseases of Aquatic Organisms·Jesse L BrunnerAllan P Pessier
Feb 23, 2019·Scientific Reports·Rosa CasaisAna Balseiro
Feb 26, 2016·Diseases of Aquatic Organisms·Betsie B RothermelMichael J Yabsley
Dec 20, 2018·Journal of Wildlife Diseases·Brittany A MosherEvan H Campbell Grant
Nov 5, 2021·Diseases of Aquatic Organisms·Gemma PalomarMaciej Pabijan

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