PMID: 9450331Feb 5, 1998Paper

Reliability of phases retrieved from 400-kV spot-scan images of purple membranes acquired on a slow-scan CCD camera

Journal of Microscopy
M B Sherman, W Chiu

Abstract

Glucose-embedded purple membranes were used as a test specimen to evaluate the reliability of phases retrieved from 400-kV spot-scan images acquired on a 1024 x 1024 slow-scan CCD camera. This specimen was chosen because it represents a broad class of low-contrast radiation-sensitive biological objects and its structure is well established. The amplitudes of computed reflections from these images were strongly damped by the modulation transfer function of the camera. Nevertheless, their phases on average were < 12 degrees different from the reference data of Henderson et al. (1986), Ultramicroscopy, 19, 147-178, up to 8.8 A resolution, which corresponds to 0.8 of the Nyquist frequency of the camera.

Citations

Jun 15, 2004·Journal of Structural Biology·Christopher R BoothWah Chiu
Jan 27, 1999·Ultramicroscopy·K H Downing, F M Hendrickson
Apr 12, 2008·Current Opinion in Structural Biology·Z Hong Zhou
Aug 7, 2004·Occupational Therapy International·Stephanie Wai-Shan Louie
May 18, 2000·Microscopy Research and Technique·P J Koeck

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