Reliable and reusable whole polypropylene plastic microfluidic devices for a rapid, low-cost antimicrobial susceptibility test

Lab on a Chip
Han SunKangning Ren

Abstract

Using an antimicrobial susceptibility test (AST) as an example, this work demonstrates a practical method to fabricate microfluidic chips entirely from polypropylene (PP) and the benefits for potential commercial use. Primarily caused by the misuse and abuse of antibiotics, antimicrobial resistance (AMR) is a major threat to modern medicine. The AST is a promising technique to help with the optimal use of antibiotics for reducing AMR. However, current phenotypic ASTs suffer from long turnaround time, while genotypic ASTs suffer from low reliability, and both are unaffordable for routine use. New microfluidics based AST methods are rapid but still unreliable as well as costly due to the PDMS chip material. Herein, we demonstrate a convenient method to fabricate whole PP microfluidic chips with high resolution and fidelity. Unlike PDMS chips, the whole PP chips showed better reliability due to their inertness; they are solvent-compatible and can be conveniently reused and recycled, which largely decreases the cost, and are environmentally friendly. We specially designed 3D chambers that allow for quick cell loading without valving/liquid exchange; this new hydrodynamic design satisfies the shear stress requirement for on-chip bac...Continue Reading

References

Jan 14, 2000·Electrophoresis·J C McDonaldG M Whitesides
Dec 3, 2004·Applied and Environmental Microbiology·Alexander H RickardPeter Gilbert
Jul 4, 2006·American Journal of Infection Control·Fred C Tenover
Jul 28, 2006·Nature·George M Whitesides
Jan 5, 2007·Lab on a Chip·Michael W Toepke, David J Beebe
Oct 2, 2007·Nature Materials·Nak Won ChoiAbraham D Stroock
Oct 28, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·James H Jorgensen, Mary Jane Ferraro
Jan 9, 2010·Analytical Chemistry·Chia Hsiang ChenPak Kin Wong
Sep 16, 2010·Lab on a Chip·Ingmar Peitz, Rien van Leeuwen
May 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kangning RenHongkai Wu
Nov 19, 2011·Drug Discovery Today·Amir M GhaemmaghamiAli Khademhosseini
Dec 1, 2011·Electrophoresis·Jinwen ZhouNicolas H Voelcker
Aug 8, 2012·Nature Reviews. Genetics·Xavier DidelotDerrick W Crook
Mar 15, 2013·Journal of Clinical Microbiology·Alex van Belkum, W Michael Dunne
Apr 19, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Robin Patel
Jul 3, 2013·The Journal of Antimicrobial Chemotherapy·Marina R PulidoMichael J McConnell
Nov 20, 2013·Accounts of Chemical Research·Kangning RenHongkai Wu
Nov 21, 2013·The Lancet Infectious Diseases·Ramanan LaxminarayanOtto Cars
Feb 4, 2014·Current Opinion in Biotechnology·Kangning RenHongkai Wu
Feb 7, 2014·Journal of Clinical Microbiology·N C GordonT Golubchik
Mar 14, 2014·Nature·Eric K SackmannDavid J Beebe
Dec 19, 2014·Science Translational Medicine·Jungil ChoiSunghoon Kwon
Feb 11, 2016·PloS One·Chih-Yao ChungHan-Sheng Chuang
Aug 10, 2017·Proceedings of the National Academy of Sciences of the United States of America·Özden BaltekinJohan Elf
Mar 16, 1998·Angewandte Chemie·Younan Xia, George M Whitesides
Nov 27, 2018·Analytical Chemistry·Morteza AziziAlireza Abbaspourrad

❮ Previous
Next ❯

Citations

Mar 30, 2021·Progress in Polymer Science·Xuan MuDavid L Kaplan
May 1, 2021·Nanomaterials·Rui VitorinoVáclav Kašička
May 26, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuta KozukaMadoka Takai
Jun 3, 2021·Micromachines·Roberts RimsaArturs Abols
May 20, 2020·ACS Applied Materials & Interfaces·Qi ZhangMark E Meyerhoff
Nov 11, 2020·Analytical Chemistry·Simon F BerlandaPetra S Dittrich
Nov 14, 2019·Analytical Chemistry·Jacob B NielsenAdam T Woolley
Dec 1, 2020·ACS Nano·Yuliang XieTony Jun Huang
Oct 5, 2021·Analytical Methods : Advancing Methods and Applications·Kotaro MoritaNaoki Hirayama

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
chips
chip

Software Mentioned

ImageJ
AutoCAD
COMSOL
COMSOL Multiphysics

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.