Reliable blood cancer cells' telomere length evaluation by qPCR

Cancer Medicine
Joana RopioEdith Chevret

Abstract

Telomere shortening is linked to a range of different human diseases, hence reliable measurement methods are needed to uncover such associations. Among the plethora of telomere length measurement methods, qPCR is reported as easy to conduct and a cost-effective approach to study samples with low DNA amounts. Cancer cells' telomere length was evaluated by relative and absolute qPCR methods. Robust and reproducible telomere length measurements were optimized taking into account a careful reference gene selection and by knowing the cancer cells ploidy. qPCR data were compared to "gold standard" measurement from terminal restriction fragment (TRF). Our study provides guidance and recommendations for accurate telomere length measurement by qPCR in cancer cells, taking advantage of our expertise in telomere homeostasis investigation in primary cutaneous T-cell lymphomas. Furthermore, our data emphasize the requirement of samples with both, high DNA quality and high tumor cells representation.

References

Mar 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K KaltoftK Thestrup-Pedersen
Apr 18, 1991·Nature·E H Blackburn
Sep 23, 1997·BioTechniques·J E BryantJ K Griffith
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·J W Shay, W E Wright
May 10, 2002·Nucleic Acids Research·Richard M Cawthon
Apr 3, 2004·Cancer Research·J Arturo Londoño-VallejoRoger R Reddel
Sep 17, 2005·Genes & Development·Titia de Lange
Jun 22, 2007·Nature·Ramiro E Verdun, Jan Karlseder
Jun 23, 2007·Genes, Chromosomes & Cancer·Martina ProchazkovaJean-Philippe Merlio
Dec 17, 2009·The New England Journal of Medicine·Rodrigo T Calado, Neal S Young
Aug 25, 2010·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Oumar SamassekouJu Yan
Jan 1, 2001·Methods in Molecular Medicine·R Roylance
Mar 4, 2011·Biological Procedures Online·Nathan J O'Callaghan, Michael Fenech
Mar 12, 2011·Seminars in Cell & Developmental Biology·Xiao Fang, Pumin Zhang
Apr 7, 2011·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Ingrid M WentzensenSharon A Savage
Apr 14, 2012·F1000 Medicine Reports·Rodrigo Calado, Neal Young
Jun 12, 2012·Aging·Elsa Vera, Maria A Blasco
May 8, 2013·The FEBS Journal·Chiou Mee KongXueying Wang
Jul 1, 2014·Nursing Research·Alison J MontpetitColleen K Jackson-Cook
Sep 23, 2014·International Journal of Epidemiology·Carmen M Martin-RuizThomas von Zglinicki
Apr 3, 2015·Methods in Ecology and Evolution·Daniel H NusseyPat Monaghan
May 20, 2015·Human Genetics·Jennifer H BarrettKaren A Pooley
Feb 27, 2016·Scientific Reports·Xun ZhuGuangfu Jin
Apr 1, 2016·Cancer Discovery·Jerry W Shay
Aug 15, 2017·Molecular Ecology Resources·Justin R EastwoodAnne Peters
Jan 18, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Tsung-Po LaiJerry W Shay
Mar 25, 2018·Neoplasia : an International Journal for Oncology Research·Paige N DahlgrenHiromi Tanaka
May 18, 2018·F1000Research·Sharon A Savage

❮ Previous
Next ❯

Citations

Jul 16, 2021·The Journal of Clinical Endocrinology and Metabolism·Richard S LeeGary S Wand
Dec 22, 2020·ACS Pharmacology & Translational Science·Tomas GoncalvesKazunori Tomita

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
density gradient centrifugation
Fluorescence
mFISH
Assay

Software Mentioned

Metafer
Ikaros
ISIS
mFISH
MxPro
QPCR
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved