Reliable eDNA detection and quantification of the European weather loach (Misgurnus fossilis).

Journal of Fish Biology
Rein BrysJoachim Mergeay

Abstract

The European weather loach (Misgurnus fossilis) is a cryptic and poorly known fish species of high conservation concern. The species is experiencing dramatic population collapses across its native range to the point of regional extinction. Although environmental DNA (eDNA)-based approaches offer clear advantages over conventional field methods for monitoring rare and endangered species, accurate detection and quantification remain difficult and quality assessment is often poorly incorporated. In this study, we developed and validated a novel digital droplet PCR (ddPCR) eDNA-based method for reliable detection and quantification, which allows accurate monitoring of M. fossilis across a number of habitat types. A dilution experiment under laboratory conditions allowed the definition of the limit of detection (LOD) and the limit of quantification (LOQ), which were set at concentrations of 0.07 and 0.14 copies μl-1 , respectively. A series of aquarium experiments revealed a significant and positive relationship between the number of individuals and the eDNA concentration measured. During a 3 year survey (2017-2019), we assessed 96 locations for the presence of M. fossilis in Flanders (Belgium). eDNA analyses on these samples highli...Continue Reading

References

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Jul 14, 2017·Biomolecular Detection and Quantification·Amin ForootanMikael Kubista
Nov 10, 2017·Molecular Ecology Resources·Robert M Dorazio, Richard A Erickson

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Citations

Feb 12, 2021·Journal of Fish Biology·Bernd Hänfling, Lori Lawson-Handley
Jul 26, 2021·The Science of the Total Environment·Beilun ZhaoKrijn Trimbos

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