Rem inhibits skeletal muscle EC coupling by reducing the number of functional L-type Ca2+ channels

Biophysical Journal
R A BannisterK G Beam

Abstract

In skeletal muscle, the L-type voltage-gated Ca(2+) channel (1,4-dihydropyridine receptor) serves as the voltage sensor for excitation-contraction (EC) coupling. In this study, we examined the effects of Rem, a member of the RGK (Rem, Rem2, Rad, Gem/Kir) family of Ras-related monomeric GTP-binding proteins, on the function of the skeletal muscle L-type Ca(2+) channel. EC coupling was found to be weakened in myotubes expressing Rem tagged with enhanced yellow fluorescent protein (YFP-Rem), as assayed by electrically evoked contractions and myoplasmic Ca(2+) transients. This impaired EC coupling was not a consequence of altered function of the type 1 ryanodine receptor, or of reduced Ca(2+) stores, since the application of 4-chloro-m-cresol, a direct type 1 ryanodine receptor activator, elicited myoplasmic Ca(2+) release in YFP-Rem-expressing myotubes that was not distinguishable from that in control myotubes. However, YFP-Rem reduced the magnitude of L-type Ca(2+) current by approximately 75% and produced a concomitant reduction in membrane-bound charge movements. Thus, our results indicate that Rem negatively regulates skeletal muscle EC coupling by reducing the number of functional L-type Ca(2+) channels in the plasma membrane.

References

Jun 23, 1972·Biochimica Et Biophysica Acta·C M ArmstrongP Horowicz
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·R G GreggP A Powers
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·M GrabnerK G Beam
Mar 29, 2000·The Journal of General Physiology·G Avila, R T Dirksen
Apr 17, 2002·The Journal of Cell Biology·Yvona WardKathleen Kelly
Nov 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Brian S FinlinDouglas A Andres
Jul 10, 2004·Circulation Research·Mitsushige MurataEduardo Marbán
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·C PaoliniC Franzini-Armstrong
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Gennady CherednichenkoIsaac N Pessah
Feb 25, 2005·The Journal of Biological Chemistry·Brian S FinlinDouglas A Andres
Sep 6, 2005·Biochemical and Biophysical Research Communications·R A Bannister, K G Beam
Oct 21, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Huanmian ChenStephen R Ikeda
Nov 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Johann SchredelsekerManfred Grabner
Jun 23, 2006·The Journal of Biological Chemistry·Brian S FinlinDouglas A Andres
Nov 1, 2006·The Journal of General Physiology·Lillian Seu, Geoffrey S Pitt
Dec 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·David C SheridanClaudio F Perez

❮ Previous
Next ❯

Citations

Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mingming FanJian Yang
Jan 13, 2015·Science China. Life Sciences·Zafir BuraeiJian Yang
Aug 12, 2009·Aging Cell·Jackson R TaylorOsvaldo Delbono
Mar 17, 2015·Pflügers Archiv : European journal of physiology·Donald BeqollariRoger A Bannister
Oct 16, 2012·Biochimica Et Biophysica Acta·Tingting Yang, Henry M Colecraft
May 31, 2015·Developmental Biology·M Gartz Hanson, Lee A Niswander
Jun 17, 2015·The Journal of General Physiology·Donald BeqollariRoger A Bannister
Mar 18, 2015·The Journal of General Physiology·Joshua D OhrtmanKurt G Beam
Jun 28, 2016·PloS One·Vinicius Henrique da SilvaLuiz Lehmann Coutinho
Oct 6, 2009·Journal of Muscle Research and Cell Motility·R A Bannister, K G Beam
Oct 14, 2011·Journal of Muscle Research and Cell Motility·Christopher L-H HuangJames A Fraser
Oct 21, 2010·Physiological Reviews·Zafir Buraei, Jian Yang
Dec 6, 2014·Channels·Viktor Yarotskyy, Robert T Dirksen
Jul 15, 2021·Cell Calcium·Daniel R MirandaRoger A Bannister

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.