Remarkably enhanced stability and function of core/shell nanoparticles composed of a lecithin core and a pluronic shell layer by photo-crosslinking the shell layer: in vitro and in vivo study

Acta Biomaterialia
Won Il ChoiGiyoong Tae

Abstract

A core/shell nanoparticle system with a lecithin core and a pluronic shell has been previously reported, and it was shown to act as an effective sustained release system for positively charged proteins. Here, to provide improved stability of the core/shell nanoparticle system in a physiological environment, we prepared the core/shell nanoparticle system with a photo-crosslinked shell layer by using a lecithin liposome as the core and pluronic F 127 diacrylate (DA-PF 127) as the shell layer. The DA-PF 127 was then photo-polymerized. Compared with a purely physical system, chemical crosslinking of the shell layer resulted not only in significantly increased structural stability of the core/shell nanoparticles in both an organic co-solvent and in serum but also several remarkably enhanced functioning as a protein delivery system. First, the chemically crosslinked systems were resuspended in aqueous solution after lyophilization without using a cryo-protectant. Second, target proteins were efficiently loaded into the nanoparticles by simple co-incubation in aqueous solution at a low temperature (4 degrees C) and the dried powder form of the protein-loaded nanoparticles was obtained. The loading capacity of the system was increased ...Continue Reading

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May 14, 2011·Expert Opinion on Drug Delivery·Zhenqing Li, Jianjun Guan
Sep 17, 2013·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ta-Chun KaoShih-Tien Hsu
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Sep 28, 2018·Expert Opinion on Drug Delivery·Andrew M Bodratti, Paschalis Alexandridis
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