Remodeling of ECM patch into functional myocardium in an ovine model: A pilot study

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Brandi B ScullyDavid L S Morales

Abstract

Previous studies have demonstrated that surgical patches comprised of small intestinal submucosa-derived extracellular matrix (ECM) have biological remodeling potential. This pilot study investigated histological, mechanical, and bioelectrical properties of an ECM patch implanted in the ovine right-ventricular outflow tract (RVOT). ECM patches (2 × 2 cm2 ) were implanted in four Western Range sheep (wether males, 37-49 kg, age <1 year) and explanted at 5 months (n = 2) and 8 months (n = 2). In vivo analysis included epicardial echocardiography and contact electrical mapping. Optical mapping was used to map electrical activity of two hearts on a Langendorff preparation. Mechanical testing quantified stiffness. Histological stains characterized structure, neovascularization, and calcification; immunohistochemistry (IHC) assessed cell phenotype. In vivo analysis showed that ECM patch tissue was contractile by M-mode and two-dimensional echocardiographic evaluation. In vivo electrical mapping, and optical mapping confirmed that ECM conducted an organized electrical signal. Mechanical testing of native and ECM patched RVOT tissue showed an elastic modulus of the implanted patch comparable to native tissue stiffness. At 5 and 8 month...Continue Reading

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Citations

Dec 21, 2016·Cardiovascular Engineering and Technology·Sharan RamaswamyFrank Scholl
Oct 20, 2017·Interactive Cardiovascular and Thoracic Surgery·Olga N KislitsinaJames L Cox
Feb 5, 2019·Advanced Healthcare Materials·Donald Bejleri, Michael E Davis
Aug 29, 2018·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·R Scott BakerDavid L S Morales
Aug 23, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Guangxiu CaoXiaoming Li
Nov 17, 2020·Frontiers in Cardiovascular Medicine·Dillon K JarrellJeffrey G Jacot

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