Remodelling of human osteoarthritic cartilage by FGF-2, alone or combined with Sox9 via rAAV gene transfer.

Journal of Cellular and Molecular Medicine
Magali CucchiariniHenning Madry

Abstract

Compensating for the loss of extracellular cartilage matrix, as well as counteracting the alterations of the chondrocyte phenotype in osteoarthritis are of key importance to develop effective therapeutic strategies against this disorder. In the present study, we analysed the benefits of applying a potent gene combination to remodel human osteoarthritic (OA) cartilage. We employed the promising recombinant adeno-associated virus (rAAV) vector to deliver the mitogenic fibroblast growth factor 2 (FGF-2) factor, alone or simultaneously with the transcription factor Sox9 as a key activator of matrix synthesis, to human normal and OA articular chondrocytes. We evaluated the effects of single (FGF-2) or combined (FGF-2/SOX9) transgene expression upon the regenerative activities of chondrocytes in three dimensional cultures in vitro and in cartilage explants in situ. Single overexpression of FGF-2 enhanced the survival and proliferation of both normal and OA chondrocytes, without stimulating the matrix synthetic processes in the increased pools of cells. The mitogenic properties of FGF-2 were maintained when SOX9 was co-overexpressed and concomitant with an increase in the production of proteoglycans and type-II collagen, suggesting th...Continue Reading

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Citations

Nov 15, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Magali CucchiariniHenning Madry
Sep 17, 2013·Journal of Translational Medicine·Jagadeesh K VenkatesanMagali Cucchiarini
Jun 16, 2011·Croatian Medical Journal·Henning Madry, Magali Cucchiarini
Oct 15, 2014·Calcified Tissue International·Bingjiang XiaPeijian Tong
Sep 4, 2014·Current Rheumatology Reports·Henning Madry, Magali Cucchiarini
Sep 4, 2014·Current Rheumatology Reports·Magali Cucchiarini, Henning Madry
Nov 26, 2015·Expert Opinion on Biological Therapy·Henning Madry, Magali Cucchiarini
Jan 26, 2016·Journal of Cellular and Molecular Medicine·Janina FrischMagali Cucchiarini
Sep 29, 2015·Tissue Engineering. Part B, Reviews·Gun-Il Im
Dec 20, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Shuiliang ShiStephen B Trippel
Sep 6, 2013·The Journal of Gene Medicine·Henning Madry, Magali Cucchiarini
Apr 11, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Lynne BarrFrances M D Henson
Feb 25, 2015·Journal of Cellular Biochemistry·Shuiliang ShiStephen B Trippel
Feb 26, 2016·Journal of Experimental Orthopaedics·Magali CucchiariniHenning Madry
Jun 9, 2016·Stem Cells and Development·Janina Frisch, Magali Cucchiarini
Mar 13, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ke TaoMagali Cucchiarini
Oct 8, 2015·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M Simental-MendíaH G Martínez-Rodríguez
May 12, 2009·Journal of Cellular and Molecular Medicine·Wei Seong TohTong Cao
Jun 20, 2020·Biomedical Materials·Gülseren Irmak, Menemse Gumusderelioglu
Jul 1, 2011·Cartilage·Henning MadryMagali Cucchiarini
Sep 29, 2020·Biomedical Materials·Gülseren Irmak, Menemşe Gümüşderelioğlu
Jan 1, 2021·Ageing Research Reviews·Linli ZhengAli Mobasheri
Feb 22, 2021·Drug Delivery and Translational Research·Héctor Rilo-AlvarezMarcos Garcia-Fuentes

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