Removal enactment of organo-phosphorous pesticide using bacteria isolated from domestic sewage

Bioresource Technology
Md ShabbirSamar K Saha

Abstract

Three bacteria (MS I, II and III) i.e., Pseudomonas aeruginosa (KY781886), Enterobactor ludwigii (KX881423) and Enterobacter cloacae (KX881513) isolated from domestic sewage were identified on the basis of 16S rDNA sequencing and are capable to growth in the presence of organo-phosphorous pesticide (chlorpyrifos). The mega plasmid size >23 kb was found in MS I and III. Biosurfactants of the significant amount were produced by three isolates. The ability of the isolates to degrade pesticide over 3 days in the presence of pesticides containing chlorpyrifos as the active component was estimated. Results of UV-visible, FTIR spectroscopy and GC-MS studies confirmed the removal of chlorpyrifos rather than degradation. Pesticide uptake results showed chlorpyrifos in intracellular components and bound to the cell surface in its native state. Removal of pesticide from soil was also recorded by these bacteria. Microbial treated pesticide did not have any effect on Vigna radita seedlings and goat erythrocytes.

Citations

Aug 23, 2019·Water Environment Research : a Research Publication of the Water Environment Federation·Jianmin WangChin-Pao Huang
Jul 5, 2021·Journal of Hazardous Materials·Kha Mong TranDokyun Na

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