PMID: 9557881Apr 29, 1998Paper

Removal of 106 amino acids from the N-terminus of UDP-GlcNAc: alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I does not inactivate the enzyme

Glycoconjugate Journal
M SarkarH Schachter

Abstract

UDP-GlcNAc: alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I (GnTI, EC 2.4.1.101) plays an essential role in the conversion of oligomannose to complex and hybrid N-glycans. Rabbit GnTI is 447 residues long and has a short four-residue N-terminal cytoplasmic tail, a 25-residue putative signal-anchor hydrophobic domain, a stem region of undetermined length and a large C-terminal catalytic domain, a structure typical of all glycosyltransferases cloned to date. Comparison of the amino acid sequences for human, rabbit, mouse, rat, chicken, frog and Caenorhabditis elegans GnTI was used to obtain a secondary structure prediction for the enzyme which suggested that the location of the junction between the stem and the catalytic domain was at about residue 106. To test this hypothesis, several hybrid constructs containing GnT I with N- and C-terminal truncations fused to a mellitin signal sequence were inserted into the genome of Autographa californica nuclear polyhedrosis virus (AcMNPV), Sf9 insect cells were infected with the recombinant baculovirus and supernatants were assayed for GnTI activity. Removal of 29, 84 and 106 N-terminal amino acids had no effect on GnTI activity; however, removal of a further 14 amino acids...Continue Reading

Citations

May 24, 2008·Plant Biotechnology Journal·Christophe SourrouilleVéronique Gomord
Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·S PagnyV Gomord
Apr 20, 2007·Proteomics·Daniel KolarichFriedrich Altmann
Dec 29, 1998·The Journal of Biological Chemistry·S ChenH Schachter
Jan 15, 2004·The Journal of Biological Chemistry·Charlotte JeanneauChristelle Breton
Jan 20, 2005·The Journal of Biological Chemistry·Kathrin MüntenerAntonio Baici
Aug 12, 1999·Biochemical and Biophysical Research Communications·H BakkerD Bosch
Nov 3, 2001·Biochimica Et Biophysica Acta·V Vallejo-RuizA Harduin-Lepers
May 29, 2003·Biochimie·Monika BencúrováIain B H Wilson
Aug 8, 2007·Protein Expression and Purification·Alexander HillarHugh O Brodovich

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