Removal of alpha-Gal epitopes from porcine aortic valve and pericardium using recombinant human alpha galactosidase A.

Journal of Korean Medical Science
Seongsik ParkYong-Jin Kim

Abstract

It has been reported that the immune response due to alpha-Gal epitopes is an important factor in tissue valve failure. The elimination of the interaction between the natural anti-Gal antibodies and alpha-gal epitopes on the xenografts is a prerequisite to the success of xenografts in humans. Previously, we reported that the green coffee bean alpha-galactosidase could remove all alpha-Gal epitopes from cell surface of porcine aortic valve and pericardial tissue, but it has limitations on cost effectiveness. In this study we wanted to know whether the recently produced recombinant human alpha-galactosidase A has the same effective enzymatic activity as green coffee bean alpha-galactosidase in removing alpha-Gal epitopes from the same tissues. After treating fresh porcine aortic valve and pericardial tissue with recombinant alpha-galactosidase A, each sample was stained with Griffonia simplicifolia type I isolectin B4 indirect immunoperoxidase avidin-biotin technique. We then examined whether the alpha-Gal epitopes were reduced or abolished in each consecutive concentration of recombinant alpha-galactosidase A by comparing the degree of the Griffonia simplicifolia isolectin B4 staining. As a result, the recombinant alpha-galactos...Continue Reading

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Citations

Jun 21, 2011·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Hong-Gook LimYong Jin Kim
Dec 19, 2013·Future Cardiology·Alice Le Huu, Dominique Shum-Tim
Mar 5, 2014·International Journal of Cardiology·Gi Beom KimSaeromi Jeong
Jul 4, 2015·Tissue Engineering. Part C, Methods·Hong-Wei GaoFeng Gong
Aug 23, 2012·Xenotransplantation·Filippo NasoGino Gerosa
Jul 20, 2010·Xenotransplantation·Mårten K J Schneider, Jörg D Seebach
Feb 4, 2014·Acta Biomaterialia·Maelene L Wong, Leigh G Griffiths
Oct 16, 2014·Tissue Engineering. Part B, Reviews·Uri Galili
Mar 18, 2017·Expert Opinion on Biological Therapy·Esmond LeeMark W Lowdell
Sep 12, 2017·Transplant International : Official Journal of the European Society for Organ Transplantation·Paolo CravediGiuseppe Orlando
Dec 19, 2017·Stem Cells International·Laura IopGino Gerosa
Feb 23, 2020·Biotechnology Journal·Ekaterina A GrebenikPeter S Timashev
Jun 13, 2018·Scientific Reports·S A H de VriesK Ito
Sep 11, 2020·Nature Reviews. Cardiology·Emanuela S FiorettaMaximilian Y Emmert
Oct 20, 2015·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Jae-Woong ChoiYong Jin Kim
Dec 30, 2020·Scandinavian Journal of Immunology·Sabin J BozsoJeevan Nagendran
Nov 23, 2020·Cell and Tissue Banking·Raziyeh KheirjouLeila Roshangar
Mar 20, 2021·Signal Transduction and Targeted Therapy·Kieran JoyceAbhay Pandit

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