Removal of detergents from protein digests for mass spectrometry analysis.

Analytical Biochemistry
Yee-Guide YeungE Richard Stanley

Abstract

Detergents are commonly used for the extraction of hydrophobic proteins and must be removed for sensitive detection of peptides by mass spectrometry. We demonstrate that ethyl acetate is able to extract octylglycoside from a protease digest without loss of peptides or interference with the peptide mass spectral profile. Ethyl acetate extraction was also found to reduce interference by sodium dodecyl sulfate, Nonidet P-40, or Triton X-100 in the mass spectrometry analysis.

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Citations

Jan 8, 2013·Analytical and Bioanalytical Chemistry·Lee Suan ChuaGiek Far Chan
May 28, 2009·Glycobiology·Jennifer T AguilanPamela Stanley
Jan 6, 2012·Journal of Lipid Research·Yusuke Suzuki, Kazuya Kabayama
Feb 11, 2015·International Journal of Molecular Sciences·Peter Feist, Amanda B Hummon
Dec 25, 2015·SpringerPlus·Benjamin Yii Chung LauUmi Salamah Ramli
Oct 21, 2011·Journal of Mass Spectrometry : JMS·Mridul Kanti MandalKenzo Hiraoka
Jun 7, 2011·Analytical Biochemistry·Babu S AntharavallyNavid Haghdoost
May 14, 2011·Analytical Biochemistry·Tatjana SajicEmmanuel Varesio
Feb 5, 2013·Journal of Proteomics·Amit ShresthaLynn A Megeney
Dec 3, 2013·Analytical Biochemistry·Tainah Silva GaldinoDário Eluan Kalume
Nov 27, 2014·Analytical and Bioanalytical Chemistry·Stefan OpitzHeidrun Rhode
Jul 21, 2016·Bioprocess and Biosystems Engineering·Reihaneh Mirhassani, Ehsan Seyedjafari
Nov 3, 2016·Scientific Reports·Siri HildonenLéon Reubsaet
Feb 26, 2015·Molecular & Cellular Proteomics : MCP·Gilles SchnellLaurence Ehret-Sabatier
Sep 4, 2015·Current Protocols in Chemical Biology·Victoria E HedrickLake N Paul
Nov 30, 2013·Analytical Methods : Advancing Methods and Applications·Leigh A WestonAmanda B Hummon
Aug 8, 2019·Mass Spectrometry Reviews·Irena DapicGarry L Corthals
Feb 26, 2020·Critical Reviews in Microbiology·Arlieke GitselsDaisy Vanrompay
May 4, 2016·Analytical and Bioanalytical Chemistry·Elizabeth H PeuchenNorman J Dovichi
Aug 2, 2012·Current Protocols in Protein Science·Babu S Antharavally
Sep 8, 2016·Proteome Science·Soundharrajan IlavenilKyung Hun Park
Feb 16, 2010·Current Protocols in Protein Science·Yee-Guide Yeung, E Richard Stanley
Jul 6, 2018·Analytical and Bioanalytical Chemistry·Monica M Schroll, Amanda B Hummon
Jun 8, 2013·The Journal of Biological Chemistry·Sayan NandiE Richard Stanley
Feb 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Carol V Robinson
Aug 14, 2019·International Journal of Molecular Sciences·Parkyong SongJong Hyuk Yoon
Jan 14, 2021·International Journal of Molecular Sciences·Yi-Hsun HoRong Huang
Jun 26, 2021·STAR Protocols·Wankyu LeeUthpala Seneviratne
Sep 12, 2017·Chemical Research in Toxicology·Alicia J DaffernerOksana Lockridge
Jun 12, 2021··Uthpala SeneviratneChristopher W. am Ende

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