PMID: 43579Oct 1, 1979

Removal of endogenous ligands from a high-affinity antiserum for radioimmunoassay

Scandinavian Journal of Clinical and Laboratory Investigation
V Kruse

Abstract

A method for removal of endogenous ligands from high-affinity antisera (stripping) is described. A thyroxine antiserum of very high affinity was used to develop the method. The antiserum was incubated at 50 degrees C in a glutamate buffer at pH 4.4 together with some ethanol and methyl cellulose and a large amount of activated charcoal. After incubation for up to 2 days, the stripped antibodies were separated from the ligand adsorbed to the charcoal by centrifugation. The estimated titre increased several fold by the stripping, although the stripping method caused a loss of about 13% of the total number of binding sites over a period of 2 days. When stripped antiserum was used instead of unstripped antiserum in an assay system, the sensitivity was up to 3 times better, and the concentration, which could be measured with the best relative precision, was 3 times lower. The stripped antiserum showed a poorer specificity than the unstripped antiserum when a short incubation period was used. However, when a long incubation period was used, the specificity was nearly the same.

References

Jan 1, 1980·Journal of Immunological Methods·R K HindawiD Riad-Fahmy
Mar 11, 1983·Journal of Immunological Methods·R Grønholt, J J Holst

Citations

Apr 1, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·J J Pratt, M G Woldring
Mar 6, 1975·Nature·F Fyhrquist, M Wallenius
Apr 1, 1977·Scandinavian Journal of Clinical and Laboratory Investigation·V Kruse, O Lind
Jan 1, 1977·Journal of Immunological Methods·P SkrabanekD Powell
Jan 1, 1976·Scandinavian Journal of Clinical and Laboratory Investigation·V Kruse
Jan 1, 1974·British Medical Bulletin·R P Ekins
Jun 1, 1969·The Journal of Clinical Endocrinology and Metabolism·G E Abraham

Related Concepts

Ethanol
Novothyral
Ethanol Measurement
Immune Sera
Thyroxine Measurement
Cross Reactions
Adsorption
Charcoal
Centrifugation
Antibody Avidity

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.