Removal of endotoxin from aqueous solutions by affinity membrane

Biomedical Chromatography : BMC
Wei GuoL Zhou

Abstract

Endotoxin was removed by affinity membranes with histidine immobilized as affinity ligand. Macropore cellulose membrane was prepared from filter paper by alkaline treatment and chemical crosslinking, and was used as matrix for the immobilization of affinity ligand. The matrix membrane was derived by hexamethylenediamine and activated by glutaraldehyde before histidine was immobilized. Membrane cartridges containing 40 or 80 sheets of affinity membrane were also prepared, which can be used to remove pyrogen from aqueous solutions. Using a cartridge with 40 sheets of affinity membrane, the endotoxin content in solution can be reduced to a minimum of 0.12 EU/mL.

References

Jun 24, 1983·Journal of Chromatography·S MinobeI Chibata

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Citations

Feb 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hirokazu SetoYoshiko Miura
Apr 26, 2020·Biotechnology and Bioengineering·Mason SchneierSutapa Barua
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·F B Anspach
Dec 14, 2011·ACS Applied Materials & Interfaces·Hirokazu SetoYoshiko Miura

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