PMID: 7019339Jan 1, 1981Paper

Removal of PHA from supernatants containing T-cell growth factor

Journal of Immunological Methods
J AlvarezM O De Landazuri

Abstract

Supernatants of human PBL cultures stimulated with PHA contain a lymphokine named T-cell growth factor (TCGF). To remove the contaminant PHA from these supernatants, we have designed a simple and effective method based on the binding properties of this lectin to chicken red blood cells. This procedure completely removes the functional activities of a PHA solution, both in lectin-induced cellular cytotoxicity against erythroid targets and in DNA synthesis of fresh PBL. The TCGF activity remaining in the supernatants after absorption with CRBC is dependent on the cellular concentration employed in the preparation of the supernatants. The absorption procedure does not remove significant TCGF activity in the supernatants prepared at high cellular concentrations (5 X 10(6) cells/ml), whereas in those prepared at low cellular concentrations (1 X 10(6) cells/ml) a partial loss of TCGF activity is detected.

References

Jul 19, 1979·Nature·E L Larsson, A Coutinho
Jan 1, 1980·Journal of Immunological Methods·R Fagnani, J A Braatz

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Citations

Jul 6, 1984·Journal of Immunological Methods·O Bruserud, T Moen
May 25, 1984·Journal of Immunological Methods·A GodardJ P Soulillou
Sep 13, 1988·Journal of Immunological Methods·R PatelD R Patel
Jan 1, 1985·Developmental and Comparative Immunology·D Watkins, N Cohen
Jun 1, 1988·Journal of Reproductive Immunology·C Garcia DomingoP Palomino
Jan 1, 1984·Veterinary Immunology and Immunopathology·A J DaemenG Kootstra
Sep 1, 1983·Human Immunology·R J Duquesnoy, A Zeevi
May 1, 1985·Clinical Immunology and Immunopathology·M GonzálezA López Borrasca
Apr 15, 1984·Cellular Immunology·M L Thoman, W O Weigle
Jul 1, 1992·Clinical and Experimental Immunology·N OgawaY Goto

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