PMID: 6966302Jan 1, 1980Paper

Removal of phytohemagglutinin from conditioned medium by affinity chromatography

Journal of Immunological Methods
R Fagnani, J A Braatz

Abstract

Supernatants of lymphocytes cultured with phytohemagglutinin (PHA-induced conditioned medium) are known to contain residual lectin. Studies with T cells specifically sensitized against a given antigen and maintained in culture by the T cell growth factor in conditioned medium may be hampered by the presence of PHA since the lectin could induce polyclonal activation of T cells. We developed a procedure for removing lectin from conditioned medium by affinity adsorption on porcine thyroglobulin-Sepharose. The affinity method was capable of removing detectable amounts of lectin since the mitogenic capacity for peripheral blood lymphocytes was lost after adsorption. In contrast, thyroglobulin-Sepharose adsorbed CM retained good mitogenic activity and growth-supporting capacity for human cultured T cells.

References

May 1, 1976·The Journal of Experimental Medicine·L ShouR A Good
Oct 1, 1978·The Journal of Experimental Medicine·S GillisK A Smith
Jan 1, 1979·Cellular Immunology·A M Silverstein
Mar 10, 1972·Biochemical and Biophysical Research Communications·I Matsumoto, T Osawa

❮ Previous
Next ❯

Citations

Jan 1, 1981·Journal of Immunological Methods·P J Spiess, S A Rosenberg
Jan 1, 1981·Journal of Immunological Methods·J AlvarezM O De Landazuri
Jul 6, 1984·Journal of Immunological Methods·O Bruserud, T Moen
Jan 1, 1984·Veterinary Immunology and Immunopathology·A J DaemenG Kootstra
Jul 1, 1981·Human Immunology·D J Schendel, R Wank
Dec 1, 1986·European Journal of Immunology·R M HoffmannG Riethmüller
Jun 30, 1983·Annals of the New York Academy of Sciences·C O ElsonE Machelski
Jun 1, 1982·European Journal of Immunology·A LanzavecchiaF Celada

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.