PMID: 9640624Jun 26, 1998Paper

Removal of primate xenoreactive natural antibodies by extracorporeal perfusion of pig kidneys and livers

Transplant Immunology
A AzimzadehP Wolf

Abstract

Organ perfusion is one of the possible strategies to attenuate rejection of discordant xenografts by reducing the levels of the recipient's xenoreactive natural antibodies (XNA). Its efficacy in terms of XNA removal was studied in models of primate blood or plasma perfusion through porcine kidneys or livers, with special attention to haematological consequences and potential side-effects. We first perfused the blood of rhesus monkeys through pig kidneys and livers, and demonstrated that the perfusion of a pig liver resulted in higher XNA adsorption (72 +/- 13%) than the perfusion of a pig kidney (51 +/- 25%). However, when we normalized for the weight of the perfused organs and for levels of natural antibodies in individual monkeys, livers adsorbed less antibody (1.4 +/- 0.9 U antibody/g) than kidneys (7.2 +/- 7 U antibody/g). Histological signs of rejection were observed in perfused kidneys, but not in perfused livers. A major drawback of the perfusion of blood through livers was a considerable decrease in the primates' haemoglobin and platelet levels. To avoid this, we developed a plasma liver perfusion device. This method allowed a significant improvement in the haemodynamic state of primates and was particularly effective i...Continue Reading

References

Nov 1, 1991·European Journal of Immunology·J L PlattF H Bach
Dec 1, 1990·Immunology Today·J L PlattF H Bach
May 1, 1990·Transplantation·J L PlattF H Bach
Sep 1, 1995·Nature Medicine·F H BachW W Hancock
Jun 15, 1995·Transplantation·C G AlvaradoJ L Platt
Aug 15, 1994·Transplantation·R L GellerJ L Platt
Feb 1, 1997·Transplantation Proceedings·A J TectorJ I Tchervenkov

❮ Previous
Next ❯

Citations

May 18, 1999·Xenotransplantation·H Auchincloss
Apr 19, 2011·Xenotransplantation·Bao-Ngoc H NguyenRichard N Pierson
Oct 27, 1999·The Journal of Thoracic and Cardiovascular Surgery·P MacchiariniP Dartevelle
May 1, 2007·The Journal of Thoracic and Cardiovascular Surgery·Bao-Ngoc H NguyenRichard N Pierson
Feb 1, 2000·Transplantation·N BarreauG Blancho
Mar 23, 2018·Acta Pharmacologica Sinica·Lei JingMingyao Liu
Nov 26, 1999·Biochimica Et Biophysica Acta·D H Joziasse, R Oriol

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Adrenocortical Carcinoma

Adrenocortical carcinoma (ACC) is a rare malignancy of the adrenal cortex, associated with a generally dismal prognosis owing to its aggressive behavior. Here are the latest discoveries pertaining to this disease.

Antibody-Dependent Cell Cytotoxicity

Antibody-dependent cellular toxicity refers to the lysis of a target cell by a non-sensitized effector cell of the immune system as a result of antibodies binding to the target cell membrane and engaging the Fc receptors on the immune effector cells. Find the latest research on antibody-dependent cellular toxicity here.