Removal of Staphylococcus aureus from skin using a combination antibiofilm approach

NPJ Biofilms and Microbiomes
Yi WangK Scott Phillips

Abstract

Staphylococcus aureus (S. aureus) including methicillin resistant S. aureus (MRSA) is one of the primary microorganisms responsible for surgical site infection (SSI). Since S. aureus contamination is known to originate from the skin, eradicating it on the skin surface at surgical sites is an important intervention to reduce the chance of SSIs. Here we developed and evaluated the efficacy of a combination probiotic/brush sonication strategy for skin preparation at surgical, injection and insertion sites in medicine. A 24 h biofilm on porcine skin explants was used as a worst-case scenario for the evaluation of preparation strategies. Conventional ethanol wipes achieved 0.8~2 log reduction in viable bacteria depending on how many times wiped (x4 or x6). Brush sonication or probiotic supernatant pre-treatment alone achieved a similar reduction as ethanol wipes (1.4 and 0.7~1.4 log reduction, respectively). Notably, combining sonication and probiotic pre-treatment achieved a 4 log reduction in viable bacteria. In addition, probiotic supernatant incubation times as short as 2 h achieved the full effect of this reduction in the combined strategy. These findings suggest the promising potential of combination-format skin preparation st...Continue Reading

References

Apr 1, 1979·Journal of Clinical Pathology·H A LillyM D Wilkins
Oct 1, 1986·Antimicrobial Agents and Chemotherapy·E L LarsonB E Laughon
Apr 29, 1999·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·A J MangramW R Jarvis
Dec 4, 2002·American Journal of Infection Control·Naomi P O'gradyUNKNOWN Healthcare Infection Control Practices Advisory Committee
Jan 10, 2006·Pflege·Silvia Schmid-BüchiRuud J G Halfens
Jan 30, 2008·Microbial Ecology·Anna M RomaníUrsula Obst
Nov 6, 2009·American Journal of Infection Control·John A WeigeltVikas Gupta
Sep 11, 2010·Journal of Innate Immunity·Yun Yun PangWilliam M Nauseef
Mar 17, 2011·Nature Reviews. Microbiology·Elizabeth A Grice, Julia A Segre
Feb 2, 2012·Micron : the International Research and Review Journal for Microscopy·Prachi TripathiYves F Dufrêne
Jan 1, 2011·National Journal of Maxillofacial Surgery·Pavitra RastogiRameshwari Singhal
Jan 30, 2013·Plastic and Reconstructive Surgery·Akhil K SethSeok J Hong
Sep 17, 2013·International Journal of Environmental Research and Public Health·Chang-Ro LeeSang Hee Lee
Oct 21, 2014·Patient Safety in Surgery·David M Tsai, Edward J Caterson
Nov 8, 2014·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Deverick J AndersonKeith S Kaye
Jan 15, 2015·Current Microbiology·Jakub KwiecinskiTao Jin
Sep 4, 2015·Biotechnology and Bioengineering·Saiqa I KhanWilliam G Austen
Apr 19, 2016·Applied Microbiology and Biotechnology·Victor Haruo MatsubaraLakshman P Samaranayake
Oct 30, 2016·BMC Microbiology·Tauá Alves MeloCarla Cristina Romano
Feb 9, 2017·Scientific Reports·Robert Jan LebbinkMonique Nijhuis
Oct 27, 2017·Frontiers in Microbiology·Louise K JensenHenrik E Jensen

❮ Previous
Next ❯

Citations

Oct 20, 2020·Critical Reviews in Biotechnology·Mehdi Kazemzadeh-NarbatAli Khademhosseini
Feb 2, 2021·Acta Biomaterialia·Laura SabioJose M Dominguez-Vera
Mar 7, 2021·International Journal of Environmental Research and Public Health·Conrado CarrascosaAntónio Raposo
Mar 13, 2021·Scientific Reports·Hao WangK Scott Phillips

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
scraping

Software Mentioned

Leica LAS

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Related Papers

Alabama Medicine : Journal of the Medical Association of the State of Alabama
T NagendranS Nagendran
Nederlands tijdschrift voor geneeskunde
P J Rietra
Medicina clínica
Nieves Sopena, Miquel Sabrià
© 2022 Meta ULC. All rights reserved