PMID: 3215839Dec 1, 1988Paper

Removal of the epithelium potentiates acetylcholine in depolarizing canine bronchial smooth muscle

Journal of Applied Physiology
Y Gao, P M Vanhoutte

Abstract

Experiments were designed to determine whether the airway epithelium affects the membrane potential of the underlying smooth muscle. The effect of epithelium removal (by gentle rubbing) on the responsiveness of isolated canine bronchi was studied. Simultaneous recordings of mechanical and electrical activity were made in paired circumferential strips (with and without epithelium) of third-order bronchi. Changes in tension were recorded with a force transducer, and changes in membrane potential were measured with a microelectrode. The cell membrane potential and resting tension of the bronchial smooth muscle were stable over a 150-min period and were not affected by removal of the epithelium. In the presence of antagonists at muscarinic and adrenergic receptors, the resting tension and membrane potential were comparable in preparations with and without epithelium. By contrast, the anticholinesterase, echothiophate, caused depolarization in bronchi without epithelium. Exposure to high potassium induced similar levels of depolarization and contraction in tissues with and without epithelium. No significant differences in threshold for depolarization or for mechanical activation in the membrane potential-tension relationship were no...Continue Reading

Citations

Aug 1, 1994·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D A KnightP J Thompson
Aug 2, 2006·Autonomic & Autacoid Pharmacology·R M Eglen
Jan 18, 2013·American Journal of Physiology. Cell Physiology·Paul M Vanhoutte
Apr 1, 1993·The Journal of Pharmacy and Pharmacology·Y ZhangC Advenier
Nov 1, 1989·The American Journal of Physiology·Y S Gao, P M Vanhoutte

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