Removing Lipemia in Serum/Plasma Samples: A Multicenter Study

Annals of Laboratory Medicine
María José Castro-CastroCommission on Lipoprotein and Vascular Diseases, Sociedad Española de Química Clínica

Abstract

Lipemia, a significant source of analytical errors in clinical laboratory settings, should be removed prior to measuring biochemical parameters. We investigated whether lipemia in serum/plasma samples can be removed using a method that is easier and more practicable than ultracentrifugation, the current reference method. Seven hospital laboratories in Spain participated in this study. We first compared the effectiveness of ultracentrifugation (108,200×g) and high-speed centrifugation (10,000×g for 15 minutes) in removing lipemia. Second, we compared high-speed centrifugation with two liquid-liquid extraction methods-LipoClear (StatSpin, Norwood, USA), and 1,1,2-trichlorotrifluoroethane (Merck, Darmstadt, Germany). We assessed 14 biochemical parameters: serum/plasma concentrations of sodium ion, potassium ion, chloride ion, glucose, total protein, albumin, creatinine, urea, alkaline phosphatase, gamma-glutamyl transferase, alanine aminotransferase, aspartate-aminotransferase, calcium, and bilirubin. We analyzed whether the differences between lipemia removal methods exceeded the limit for clinically significant interference (LCSI). When ultracentrifugation and high-speed centrifugation were compared, no parameter had a differenc...Continue Reading

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Citations

Jun 27, 2019·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Carolina N AzevedoUnity Jeffery
Jul 17, 2020·The Journal of Applied Laboratory Medicine·Mustafa A BarbhuiyaYusheng Zhu
Dec 29, 2020·Analytical Methods : Advancing Methods and Applications·Georgia Eleni TsotsouPetros Karkalousos
Mar 19, 2021·Annals of Clinical Biochemistry·Jun Guan Tan, Moh Sim Wong
Aug 22, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hikmet Can ÇubukçuUNKNOWN European Federation of Clinical Chemistry, Laboratory Medicine EFLM Working Group Accreditation, ISO/CEN standards WG-A/ISO
Oct 7, 2021·Biopreservation and Biobanking·Randi E GislefossMarcin W Wojewodzic
Dec 19, 2021·Scientific Reports·Justin R PerraultCharles A Manire

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Software Mentioned

SPSS
LipoClear

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