Renal ACE2 expression and activity is unaltered during established hypertension in adult SHRSP and TGR(mREN2)27

Hypertension Research : Official Journal of the Japanese Society of Hypertension
Jelena KamilicH van Goor

Abstract

Differential renal expression of a homolog of the angiotensin-converting enzyme (ACE), that is, ACE2, has been implicated as a genetic basis of polygenetic hypertension in the stroke-prone spontaneously hypertensive rat model. However, data on the role of ACE2 in hypertension are still inconclusive. Therefore, we analyzed kidney ACE2 mRNA, ACE2 protein and ACE2 enzyme activities in the adult polygenetic stroke-prone spontaneously hypertensive rat (SHRSP) and the monogenetic TGR(mREN2)27 rat models, in comparison with their normotensive reference strains, Wistar-Kyoto (WKY) and Spraque-Dawley (SD) rats, respectively. Kidney ACE2 mRNA was studied using quantitative real-time reverse transcriptase-PCR (RT-PCR) in cortex and medulla, whereas protein expression was scored semiquantitatively in detail in different renal structures using immunohistochemistry. Furthermore, total renal tissue ACE2 activity was measured using a fluorimetric assay that was specified by the ACE2 inhibitor DX600. In SHRSP and homozygous TGR(mREN2)27 rats with established hypertension, kidney ACE2 mRNA, protein and tissue ACE2 activities were not different from their respective WKY and SD reference strain, respectively. In addition, when we looked at renal l...Continue Reading

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Citations

Dec 19, 2009·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Mark C Chappell
Apr 3, 2012·Antioxidants & Redox Signaling·Kaushik P Patel, Harold D Schultz
Dec 28, 2010·Canadian Journal of Physiology and Pharmacology·Waylon WisemanMarva I Sweeney
Nov 17, 2020·Frontiers in Molecular Biosciences·Federica SaponaroRiccardo Zucchi
Apr 2, 2021·Circulation Research·Carmine SavoiaReinhold Kreutz

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