Renal acid excretion and intracellular pH in salt-sensitive genetic hypertension

The Journal of Clinical Investigation
D BatlleC Gutterman

Abstract

Acid-base status and renal acid excretion were studied in the Dahl/Rapp salt-sensitive (S) rat and its genetically salt-resistant counterpart (R). S rats developed hypertension while on a very high salt diet (8%) and while on a more physiological salt diet (1%) and remained normotensive while on a very low salt diet (0.08%). Under the high salt diet, intracellular pH measured in freshly isolated thymic lymphocytes using 2',7'-bis (carboxyethyl)-5 (6)-carboxyfluorescein acetomethyl ester, a pH-sensitive dye, was lower in S than in R rats both when measured in the presence of HCO3/CO2 (7.32 +/- 0.02 vs. 7.38 +/- 0.02, respectively, P < 0.05) and in its absence (7.18 +/- 0.04 vs. 7.27 +/- 0.02, respectively, P < 0.05). Under the high salt diet, net acid excretion was higher in S than R rats (1,777 +/- 111 vs. 1,017 +/- 73 muEq/24 h per 100 g body wt, respectively, P < 0.001), and this difference was due to higher rates of both titratable acid and ammonium excretion. Directionally similar differences in intracellular pH and net acid excretion between S and R rats were also observed in salt-restricted animals. In S and R rats placed on a normal salt intake (1%) and strictly pair-fed to control food intake as a determinant of dietary...Continue Reading

References

Dec 1, 1978·Proceedings of the Society for Experimental Biology and Medicine·G L WrightF Dukes-Dobos
Nov 1, 1991·The Journal of Clinical Investigation·P Y Chen, P W Sanders
Nov 1, 1991·The Journal of Clinical Investigation·O W MoeR J Alpern
Nov 1, 1991·Hypertension·G M ReavenH Chang
Apr 1, 1989·Journal of Hypertension·R L Schmouder, A B Weder
Oct 1, 1989·Kidney International·G A MorduchowiczN Yanagawa
Oct 22, 1987·The New England Journal of Medicine·T W KurtzR C Morris

❮ Previous
Next ❯

Citations

Feb 24, 2001·International Journal of Clinical & Laboratory Research·I PuscasA Hecht
Aug 22, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Attilio LositoGaetano Ferrara
Apr 15, 2008·Current Opinion in Nephrology and Hypertension·Khashayar Sakhaee
Dec 19, 2009·The Journal of Urology·Brian H EisnerMarshall L Stoller
Apr 25, 2006·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Eric N TaylorGary C Curhan
Jan 26, 2008·American Journal of Hypertension·Sergio H Obligado, David S Goldfarb
Mar 23, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·I PuscasA Hecht
Dec 1, 1993·Hypertension·A M SharmaA Distler
Apr 17, 2019·International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition·Manije Darooghegi MofradLeila Azadbakht
Jan 4, 2013·American Journal of Physiology. Renal Physiology·Wolfgang FreisingerRoland Veelken
Oct 30, 1998·Journal of Hypertension·D E AndersonA Y Bagrov
Feb 5, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Paul M O'ConnorChunhua Jin
Aug 11, 2001·American Journal of Hypertension·D E AndersonA Y Bagrov
Jul 29, 2017·Journal of Clinical Biochemistry and Nutrition·Akane MikiMichiaki Fukui

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.