Renal apoptosis following carbon dioxide pneumoperitoneum in a rat model

The Journal of Urology
Wisam KhouryAvi A Weinbroum

Abstract

Laparoscopically recruited kidneys regain normal function more slowly than laparotomy harvested organs for several possible reasons. We investigated the effects of CO(2) induced pneumoperitoneum on kidney function, as reflected by blood and urine creatinine levels, and its relation with renal cell apoptosis. CO(2) pneumoperitoneum was established in anesthetized Wistar male rats that were randomly allocated at 6 per group into 1 of 6 groups with an intraperitoneal pressure of 0 (control), 5, 8, 12, 15 or 18 mm Hg. Pressure was maintained for 60 minutes in all groups. Three additional groups were subjected to 30-minute pneumoperitoneum at 0, 12 and 18 mm Hg, respectively. The rats were kept alive for the ensuing 24 hours, after which blood and urine creatinine were analyzed and the abdominal organs were harvested. Various areas of the organs were analyzed for apoptotic cells using the TUNEL method. Cells were randomly counted in 10 eyeshots in 3 sections each using an ocular micrometer. Creatinine levels in blood and urine changed as pressure and pneumoperitoneum duration progressed. Isolated TUNEL positive nuclei were detected in the outer medulla and the cortex of control kidneys. There was a significantly higher number of TUN...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Apr 29, 1998·Annual Review of Physiology·E B Thompson
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·S Kiryu-SeoH Kiyama
Aug 5, 2000·Current Opinion in Nephrology and Hypertension·A M Sheridan, J V Bonventre
Apr 5, 2001·Surgical Endoscopy·J A AmbroseJ A Schulak
Apr 24, 2001·Journal of the American Society of Nephrology : JASN·Chiang-Ting ChienSu-Ming Hsu
Mar 5, 2003·Annals of Surgery·Eric J HanlyMark A Talamini
Nov 1, 1960·Anesthesiology·H L PRICE
Nov 26, 2003·Journal of the American Society of Nephrology : JASN·Akito MaeshimaYoshihisa Nojima
Sep 16, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Alexander KainzRainer Oberbauer
Sep 7, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nele KindtEdward M Conway

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Citations

Aug 16, 2015·Surgical Endoscopy·Denise M D Özdemir-van BrunschotMichiel C Warlé
Mar 16, 2013·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Diogo B de SouzaFrancisco J B Sampaio
Jul 12, 2013·Acta Cirúrgica Brasileira·Pedro Felipe Silva de FreitasJoão Batista de Sousa

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis