Renal artery injection for delivery of biological materials to the glomerulus

Nephrology
Yoshitsugu TakabatakeEnyu Imai

Abstract

We investigated whether injection of synthetic small interfering siRNAs via renal artery followed by electroporation could be effective in silencing specific genes in glomerulus. Solution of siRNA targeting enhanced green fluorescent protein (EGFP) was injected via the renal artery of EGFP-transgenic rats followed by delivery of electric pulses. The delivery of siRNA reduced endogenous EGFP expression, mainly in glomerular mesangial cells. Electroporation-mediated gene transfer system via renal artery in rats is very useful for the delivery of a range of different materials including cells, viruses and proteins. Here, we provide a step-by-step description of this perfusion method and a discussion of the key points for the success of the technique.

References

Apr 24, 2001·Journal of the American Society of Nephrology : JASN·Michiko TsujieMasatsugu Hori
Dec 1, 2001·Journal of the American Society of Nephrology : JASN·Takahito ItoMasatsugu Hori
Jun 4, 2002·Nature Medicine·Carl D NovinaPhillip A Sharp

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Citations

Oct 2, 2013·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·Marina Gabriela Monteiro Carvalho Mori da CunhaJaan Toelen

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