PMID: 3744898Jan 1, 1986Paper

Renal cathepsin-B activities in rats after castration and treatment with sex hormones

Histochemistry
P Kugler, G Vornberger

Abstract

The activities of the lysosomal endopeptidase cathepsin B (cath B; CZB-Ala-Arg-Arg-MNA as substrate) and the lysosomal exopeptidase dipeptidylpeptidase II (DAP II; Lys-Ala-2NA as substrate) were fluorometrically determined in the renal homogenate of normal and experimental (castration followed by a 14-day treatment with estradiol and testosterone) rats of both sexes. In addition, methodological investigations of the renal homogenate were performed in order to differentiate cath B from other proteinases. These showed that cath-B activity was highest at around pH 6, was strongly inhibited by 4-hydroxymercuribenzoate and leupeptin, and was activated by dithiothreitol. Trypsin-like activities were not demonstrable under the used incubation conditions. The animal experiments showed that renal cath-B activities (1) were significantly higher in females than in males, (2) increased significantly in males and decreased significantly in females after castration (no significant difference between both sexes), (3) decreased in female and male castrates after treatment with testosterone and increased strongly after treatment with estradiol, and (4) showed an activity pattern similar to that of DAP II. The results are discussed in relation t...Continue Reading

References

Oct 1, 1976·Kidney International·E I Christensen
Jan 1, 1985·Histochemistry·F Kiesewetter, P Kugler
Jan 24, 1973·Biochimica Et Biophysica Acta·W HuismanM Gruber
Nov 25, 1974·Biochimica Et Biophysica Acta·W HuismanM Gruber
Dec 1, 1974·Kidney International·E I Christensen, A B Maunsbach
Jan 1, 1983·Current Topics in Cellular Regulation·N Katunuma, E Kominami
Jan 1, 1982·Histochemistry·K Jedrzejewski, P Kugler
Aug 1, 1982·The Biochemical Journal·A D Gounaris, E E Slater
Dec 1, 1981·Journal of Biochemistry·S TakahashiY Miyake
Jan 1, 1980·Histochemistry·M DavidoffT H Schiebler

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Citations

Jan 1, 1985·Histochemistry·F Kiesewetter, P Kugler
Feb 9, 1995·Biochimica Et Biophysica Acta·D Fu, C A Hornick
Mar 3, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Marie-Berthe MaesIngrid De Meester
Nov 1, 1988·Toxicology and Applied Pharmacology·C V MurtyA K Roy

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