Renal cells activate the platelet receptor CLEC-2 through podoplanin.

The Biochemical Journal
Charita M ChristouChris A O'Callaghan

Abstract

We have recently shown that the C-type lectin-like receptor, CLEC-2, is expressed on platelets and that it mediates powerful platelet aggregation by the snake venom toxin rhodocytin. In addition, we have provided indirect evidence for an endogenous ligand for CLEC-2 in renal cells expressing HIV-1. This putative ligand facilitates transmission of HIV through its incorporation into the viral envelope and binding to CLEC-2 on platelets. The aim of the present study was to identify the ligand on these cells which binds to CLEC-2 on platelets. Recombinant CLEC-2 exhibits specific binding to HEK-293T (human embryonic kidney) cells in which the HIV can be grown. Furthermore, HEK-293T cells activate both platelets and CLEC-2-transfected DT-40 B-cells. The transmembrane protein podoplanin was identified on HEK-293T cells and was demonstrated to mediate both binding of HEK-293T cells to CLEC-2 and HEK-293T cell activation of CLEC-2-transfected DT-40 B-cells. Podoplanin is expressed on renal cells (podocytes). Furthermore, a direct interaction between CLEC-2 and podoplanin was confirmed using surface plasmon resonance and was shown to be independent of glycosylation of CLEC-2. The interaction has an affinity of 24.5+/-3.7 microM. The pre...Continue Reading

References

Feb 27, 1999·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·K MatsuiD Kerjaschki
Jul 23, 2003·Journal of the American Society of Nephrology : JASN·Klaas KoopJan A Bruijn
Aug 17, 2005·Journal of Thrombosis and Haemostasis : JTH·S P WatsonA C Pearce
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Aleksandra A Watson, Christopher A O'Callaghan
Aug 31, 2006·Journal of Virology·Chawaree ChaipanStefan Pöhlmann
Nov 30, 2006·The Journal of Biological Chemistry·Aleksandra A WatsonChristopher A O'Callaghan
Mar 7, 2007·The Journal of Biological Chemistry·Gemma L J FullerAndrew C Pearce
Jul 10, 2007·The Journal of Biological Chemistry·Katsue Suzuki-InoueYukio Ozaki

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Citations

Jul 24, 2008·Cell and Tissue Research·Giorgia Jurisic, Michael Detmar
Feb 19, 2010·The Journal of Biological Chemistry·Lakshmi C WijeyewickremaRobert K Andrews
Oct 29, 2008·The Journal of Biological Chemistry·Jun MoriSteve P Watson
Feb 24, 2010·AIDS·Claudette S SatchellPatrick W G Mallon
Nov 10, 2011·Toxins·Aleksandra A Watson, Christopher A O'Callaghan
Jan 24, 2013·The Journal of Biological Chemistry·Bhanu Kanth ManneSatya P Kunapuli
Apr 11, 2013·International Reviews of Immunology·Anthony PlatoGordon D Brown
Jul 1, 2011·Platelets·Katsue Suzuki-InoueYukio Ozaki
Nov 17, 2015·The Journal of Clinical Investigation·Jessica R HitchcockAdam F Cunningham
Jul 28, 2009·Journal of Thrombosis and Haemostasis : JTH·Y OzakiO Inoue
Jun 28, 2008·Protein Science : a Publication of the Protein Society·Aleksandra A WatsonChris A O'Callaghan
Mar 30, 2010·Journal of Thrombosis and Haemostasis : JTH·S P WatsonA Y Pollitt
Aug 4, 2011·Journal of Thrombosis and Haemostasis : JTH·K Suzuki-InoueY Ozaki
Jul 17, 2013·Journal of Thrombosis and Haemostasis : JTH·Y OzakiO Inoue
Nov 14, 2013·International Journal of Cancer. Journal International Du Cancer·Satoshi TakagiNaoya Fujita
Dec 18, 2009·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Apr 10, 2015·Platelets·Fuminori KazamaYukio Ozaki
Mar 26, 2008·Biochemical and Biophysical Research Communications·Jianhui XieJianxin Gu
Aug 12, 2009·Cytokine·Lisa M Graham, Gordon D Brown
Feb 22, 2011·Trends in Immunology·Ann M Kerrigan, Gordon D Brown
Dec 19, 2008·Current Opinion in Pharmacology·Chris A O'Callaghan
Oct 12, 2010·Immunology Letters·Pierre Redelinghuys, Gordon D Brown
Feb 16, 2010·Blood·Craig E HughesSteve P Watson
Dec 3, 2014·Biochemical Pharmacology·Bhanu Kanth ManneSatya P Kunapuli
Nov 22, 2014·The Journal of Clinical Investigation·Anneli PetersVijay K Kuchroo
Jun 5, 2018·Platelets·Katsue Suzuki-Inoue

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