Renal hemodynamic and morphological changes after 7 and 28 days of leptin treatment: the participation of angiotensin II via the AT1 receptor

PloS One
Karina Thieme, Maria Oliveira-Souza

Abstract

The role of hyperleptinemia in cardiovascular diseases is well known; however, in the renal tissue, the exact site of leptin's action has not been established. This study was conducted to assess the effect of leptin treatment for 7 and 28 days on renal function and morphology and the participation of angiotensin II (Ang II), through its AT1 receptor. Rats were divided into four groups: sham, losartan (10 mg/kg/day, s.c.), leptin (0.5 mg/kg/day for the 7 days group and 0.25 mg/kg/day for the 28 days group) and leptin plus losartan. Plasma leptin, Ang II and endothelin 1 (ET-1) levels were measured using an enzymatic immuno assay. The systolic blood pressure (SBP) was evaluated using the tail-cuff method. The renal plasma flow (RPF) and the glomerular filtration rate (GFR) were determined by p-aminohippuric acid and inulin clearance, respectively. Urinary Na+ and K+ levels were also analyzed. Renal morphological analyses, desmin and ED-1 immunostaining were performed. Proteinuria was analyzed by silver staining. mRNA expression of renin-angiotensin system (RAS) components, TNF-α and collagen type III was analyzed by quantitative PCR. Our results showed that leptin treatment increased Ang II plasma levels and progressively increas...Continue Reading

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Citations

Aug 20, 2016·The Journal of Endocrinology·Jinn-Yang ChenChi-Chang Juan
Mar 11, 2016·American Journal of Physiology. Renal Physiology·Fernando Augusto Malavazzi CasareMaria Oliveira-Souza
Sep 29, 2020·Frontiers in Physiology·Larissa de AraújoMaria Oliveira-Souza
May 27, 2021·Clinical and Experimental Pharmacology & Physiology·Carla Patrícia CarlosAgnaldo Bruno Chies

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Methods Mentioned

BETA
enzymatic immuno assay
urine collections
PCR

Software Mentioned

GraphPad Prism
NIS
Scion Image
- Elements D

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