Renal Ischemia-Reperfusion Injury in a Diabetic Monkey Model and Therapeutic Testing of Human Bone Marrow-Derived Mesenchymal Stem Cells

Journal of Diabetes Research
Kyo Won LeeSung Joo Kim

Abstract

Clinically, acute kidney injury (AKI) episodes in diabetes mellitus (DM) patients are associated with a cumulative risk of developing end-stage renal disease. In this study, we asked whether the severity of AKI induced by renal ischemia-reperfusion injury (IRI) is more prominent in DM than in non-DM control using a cynomolgus monkey (Macaca fascicularis) model. We also investigated whether human bone marrow-derived mesenchymal stem cells (hBM-MSCs) infused via the renal artery could ameliorate renal IRI in DM monkeys. The experimental data, including mortality rate, histologic findings, and urinary albumin secretion indicate that the severity of AKI was greater in DM monkeys than in control animals. Moreover, histological findings and qRT-PCR analysis of Ngal mRNA in renal biopsy tissue showed that hBM-MSC promoted the recovery of tubular damage caused by AKI. Serum analysis also revealed that the level of albumin and ALT was increased 24 and 48 hours after AKI, respectively, suggesting that AKI induced acute liver injury. We suggest that this nonhuman primate model could provide essential information about the renal and nonrenal impairment related to DM and help determine the clinical usefulness of MSCs in AKI.

References

Mar 13, 2003·Kidney International·Hamid RabbManoocher Soleimani
May 23, 2003·Journal of the American Society of Nephrology : JASN·K J Kelly
Jun 26, 2003·The Journal of Clinical Investigation·Sujata KaleLloyd G Cantley
Feb 17, 2005·American Journal of Physiology. Renal Physiology·Florian TögelChristof Westenfelder
Apr 4, 2008·Kidney International·C Y HsuA S Go
Aug 7, 2009·American Journal of Physiology. Renal Physiology·K J KellyJesus H Dominguez
Feb 26, 2010·Mediators of Inflammation·Ali AkcayCharles L Edelstein
Jan 26, 2011·Annual Review of Pathology·Yashpal S KanwarSheldon Chen
May 3, 2011·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Gilbert R Kinsey, Mark D Okusa
Sep 10, 2011·Clinical Journal of the American Society of Nephrology : CJASN·Charuhas V ThakarAnthony C Leonard
Feb 18, 2012·Annual Review of Physiology·Volker Vallon, Scott C Thomson
Mar 23, 2012·JAMA : the Journal of the American Medical Association·Jianming TanCamillo Ricordi
Sep 21, 2012·American Journal of Physiology. Renal Physiology·Qingqing Wei, Zheng Dong
Jan 4, 2013·American Journal of Physiology. Renal Physiology·Guofeng GaoW Brian Reeves
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Junhee SeokUNKNOWN Inflammation and Host Response to Injury, Large Scale Collaborative Research Program
Sep 17, 2013·Nature Reviews. Immunology·Christian KurtsAndrew J Rees
Nov 22, 2014·Proceedings of the National Academy of Sciences of the United States of America·Shin LinMichael P Snyder
Nov 27, 2014·World Journal of Stem Cells·Francesca BianchiGaetano La Manna
Oct 10, 2015·Nephrology·Faye JohnsonAled O Phillips
Nov 1, 2015·Journal of the American Society of Nephrology : JASN·Mark D OkusaUNKNOWN Acute Dialysis Quality Initiative XIII Workgroup
Nov 14, 2015·Molecular BioSystems·A Jin WonHyung Sik Kim
Jan 13, 2016·Stem Cell Research & Therapy·Johannes Leibacher, Reinhard Henschler
Jan 16, 2016·Annual Review of Medicine·Anna Zuk, Joseph V Bonventre
Dec 16, 2016·International Journal of Nephrology·D Patschan, G A Müller
Apr 28, 2017·Stem Cell Research & Therapy·Yuriy PetrenkoŠárka Kubinová
May 12, 2017·Tissue Engineering. Part B, Reviews·Yao FuMarcel Karperien
Feb 17, 2018·Journal of the American Society of Nephrology : JASN·Nils O LindströmAndrew P McMahon

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Citations

Aug 15, 2019·BMC Nephrology·Henrique Pinheiro KonigsfeldMarcelino de Souza Durão Junior
Jul 25, 2019·International Journal of Molecular Sciences·Kuo-Hua LeeDer-Cherng Tarng
Oct 13, 2020·Renal Failure·Balamurugan PackialakshmiXiaoming Zhou
Jun 10, 2021·Stem Cells Translational Medicine·LaTonya J HicksonM Hassan Murad

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Methods Mentioned

BETA
pancreatectomy
sedation
biopsy
ELISA
reverse transcription PCR
biopsies

Software Mentioned

SPSS

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