Renal membrane-bound carbonic anhydrase. Purification and properties

Kidney International
P J Wistrand, K G Knuuttila

Abstract

Microsomes from perfused human donor kidneys were separated by differential centrifugation in sucrose, and thoroughly washed before solubilization by the nonionic detergent nonyl-beta-D-glucoside. The solubilized material was first applied onto an affinity chromatographic column of acetazolamide-oxirane-SepharoseR-CL-4B to remove contaminating cytoplasmic carbonic anhydrase isozymes CA I and CA II. It was then added onto an affinity column of p-aminomethylbenzene sulfonamide coupled to CM Bio-gel AR to purify the membrane-bound carbonic anhydrase activity. This resulted in a 50% pure enzyme. It was then concentrated and fractionated on an anion-exchange column, and desalted and purified to homogeneity (SDS-PAGE and isoelectric focusing) by gel filtration. The enzyme was now purified 411-fold from extractable membrane protein. Its molecular weight was 34.4 kDa from gel filtration and SDS-PAGE, and 36.7 kDa from amino acid analysis. The amino acid composition differed from that of the cytoplasmic isozymes CA I, II, and III. Antisera, produced in rabbits against the purified SDS-treated enzyme, reacted with native nondenatured membrane enzyme protein but only weakly with CA II. Kinetically the enzyme was similar to CA II with resp...Continue Reading

References

Jun 1, 1979·Acta Physiologica Scandinavica·B KarlmarkP J Wistrand
Oct 11, 1976·Biochimica Et Biophysica Acta·D N McKinley, P L Whitney
Aug 29, 1977·Pflügers Archiv : European journal of physiology·P J Wistrand, R Kinne
Sep 1, 1975·European Journal of Biochemistry·J WistrandT Wåhlstrand
Jul 15, 1971·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S Funakoshi, H F Deutsch
May 1, 1983·Journal of Neurochemistry·V S SapirsteinJ M Gilbert
Jan 1, 1984·Annals of the New York Academy of Sciences·P J Wistrand
Jun 1, 1984·Kidney International·G Lönnerholm, P J Wistrand
Jan 1, 1980·Upsala Journal of Medical Sciences·T Wåhlstrand, P J Wistrand
Jan 15, 1981·Biochimica Et Biophysica Acta·G SanyalT H Maren
Aug 1, 1953·The Biochemical Journal·M DIXON
Feb 1, 1965·The Journal of Clinical Investigation·F C RECTORD W SELDIN

❮ Previous
Next ❯

Citations

May 1, 1990·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·J Rodríguez-Soriano, A Vallo
May 1, 1991·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·U AlonB A Warady
Feb 1, 1996·Biochemical Genetics·S TamaiW S Sly
Jul 9, 1990·Biochimica Et Biophysica Acta·N D CarterP J Wistrand
May 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A A López MañanesF V Vega
Jan 28, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Keitaro KitadeYoshiaki Obara
Jan 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Hans-Peter SchneiderJoachim W Deitmer
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S SatoW S Sly
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D BrownW S Sly
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·G S HagemanW S Sly
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M S GhandourW S Sly
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T OkuyamaW S Sly
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y NagaoW S Sly
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y NagaoW S Sly
Mar 4, 2000·Preparative Biochemistry & Biotechnology·Y DemirE Bakan
Jan 1, 1986·Acta Physiologica Scandinavica·G LönnerholmE Bárány
Feb 1, 1991·Acta Physiologica Scandinavica·G Lönnerholm, P J Wistrand
Mar 13, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nataliya SvicharMitchell Chesler
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·O TüreciW S Sly
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·T StamsD W Christianson
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·S TamaiW S Sly
Jun 30, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Ozen Ozensoy GulerClaudiu T Supuran
Jun 19, 2014·Journal of Enzyme Inhibition and Medicinal Chemistry·Murat BozdagClaudiu T Supuran
Jun 1, 1996·Kidney International·G SekiE Frömter
Oct 1, 1990·Kidney International·M Martinez-Maldonado, H R Cordova
Dec 14, 2006·Kidney International·J M Purkerson, G J Schwartz
May 12, 2009·Respiratory Physiology & Neurobiology·Andrew J EsbaughS F Perry
Mar 14, 1998·Protein Expression and Purification·J D HurtP J Laipis
Aug 21, 2012·International Journal of Biological Macromolecules·Ahmad SharifizadehReza Yousefi
Mar 25, 1999·Acta Physiologica Scandinavica·P J WistrandI Mahieu
Oct 30, 2016·International Journal of Biological Macromolecules· WahiduzzamanFaizan Ahmad
Apr 26, 2001·Preparative Biochemistry & Biotechnology·N DemirH Nadaroglu
Jan 1, 1996·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·M F Sugrue
Dec 1, 1995·The Histochemical Journal·A K ParkkilaH Rajaniemi
Sep 16, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·K M GilmourS F Perry
Jun 8, 2019·Expert Opinion on Therapeutic Patents·Sabina ZamanovaMarc A Ilies

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
D BrownW S Sly
Proceedings of the National Academy of Sciences of the United States of America
S SatoW S Sly
The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
S ParkkilaH Rajaniemi
The International Journal of Biochemistry
H F Deutsch
Proceedings of the National Academy of Sciences of the United States of America
T OkuyamaW S Sly
© 2022 Meta ULC. All rights reserved